Re: [ccp4bb] PCB buffer and MMT buffer

Hi all, I only wish to draw your attention to the fact that the Henderson-Hasselbach equation (as used in the paper you cite) is only valid if you use zwitterionic buffers. Otherwise, with other buffers, especially phosphate and multi-acids (e.g. succinate, citrate and so on), any calculation

Re: [ccp4bb] PCB buffer and MMT buffer

Hi, These buffers are available from Molecular Dimensions Ltd. and Jena Biosciences. Regards Lesley -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Behalf Of Jenny Sent: 30 January 2007 04:20 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] PCB buffer and MMT buffer Hi

Re: [ccp4bb] PCB buffer and MMT buffer

Hi Jenny (and others), The buffer systems used in the PACT screen are described in the following paper by Janet Newman. /Acta Cryst./ (2004). D*60*, 610-612[ doi:10.1107/S0907444903029640 ] Novel buffer systems for macromolecular crystallizat

[ccp4bb] PCB buffer and MMT buffer

Hi, Sorry to bother you all.I'm going to try to reproduce some results from the initial screening from PACT kit.The conditions are something like 0.1M PCB buffer, 25% w/v PEG 1500 and 0.1 MMT buffer, 25%w/v PEG 1500. I was just wondering is there any easy way to get these buffers?Do you happen to