Re: [ccp4bb] Peptide solubility issues

For that purpose we’ve developed this expression system that may or may not be useful to your specific problem: http://www.ncbi.nlm.nih.gov/pubmed/23996492 J Mol Recognit. 2013 Oct;26(10):496-500. doi: 10.1002/jmr.2292. Development of a multifunctiona

Re: [ccp4bb] Peptide solubility issues

Many thanks to all the people who contacted me. I actually went rogue and decided to initially reconstitute in 10mM glycine, pH 9.5 to 2 mg/mL, sonicate for a few seconds and then immediately add an equal volume of more concentrated neutral pH buffer to minimize any possibility of alkaline hydrolys

Re: [ccp4bb] Peptide solubility issues

Hello Mo Wong, Some points below are good, but don't underestimate custom peptides sometimes... can be much harder/expensive than recombinant proteins. If you ordered the peptides it is good to know how they synthesized them, and how the elution profiles during purification (RP HPLC..) looked like,

[ccp4bb] Peptide solubility issues

Hi all, Slightly off topic - but I'm having trouble solubilizing some peptides for SPR and hoped someone on the BB might have some other suggestions. The peptides are intra-chain S-S cross-linked 12mers with pIs of ~3. 10 residues are hydrophobic and 2 are acidic. Peptides have been tested with a