a most approapriate paper for the method that i described earlier is
in the following
paper. Follow the link
Optimum solubility (OS) screening: an efficient
method to optimize buffer conditions for
homogeneity and crystallization of proteins
http://journals.iucr.org/d/issues/2004/09/00/dz5020/dz5
On Thu, 2010-04-29 at 16:23 +0530, Jhon Thomas wrote:
> This is a strange behaviour of the protein complex.
Why is this strange? 20 mg/ml is fairly high, just dilute the protein
to 10 mg/ml and repeat the screen. Or better yet, repeat the screen
with 1:2 protein:reservoir ratio for precipitated
Hello BB
I am working with small protein-protein complex of Molecular weight 40kDa.
This protein expresses well and soluble in 20mM Tris-Cl pH-8.0 and 50mM of
KCl, could be concentrated upto 20mg per ml in this buffer. I have purifed
this protein with Ni-NTA resin and they are free from any contam
