[ccp4bb] Precipitation of protein-protein complex under most of the crystallization condition

Hello BB I am working with small protein-protein complex of Molecular weight 40kDa. This protein expresses well and soluble in 20mM Tris-Cl pH-8.0 and 50mM of KCl, could be concentrated upto 20mg per ml in this buffer. I have purifed this protein with Ni-NTA resin and they are free from any

Re: [ccp4bb] Precipitation of protein-protein complex under most of the crystallization condition

On Thu, 2010-04-29 at 16:23 +0530, Jhon Thomas wrote: This is a strange behaviour of the protein complex. Why is this strange? 20 mg/ml is fairly high, just dilute the protein to 10 mg/ml and repeat the screen. Or better yet, repeat the screen with 1:2 protein:reservoir ratio for precipitated

Re: [ccp4bb] Precipitation of protein-protein complex under most of the crystallization condition

a most approapriate paper for the method that i described earlier is in the following paper. Follow the link Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins