to:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Roger
Rowlett
Sent: Thursday, July 19, 2012 4:42 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Protein concentration vs Molecular wt...
This is almost exactly our basic approach, too. Before we got a dropsetter, we
did 24 wells (1/2 screen) to get a fee
See http://www.douglas.co.uk/PDB_data.htm, section 4
On 19 July 2012 20:58, james09 pruza wrote:
> Dear Crystallographers,
> Is there any rule of thumb for Protein concentration and molecular weight
> for crystallization trials of a soluble protein? Looking for high molecular
> wt. protein ~50
Hi James,
You can get the PCT (Pre-Crystallisation Test) from one of the more
famous manufacturers of crystallography products - essentially you can
quickly screen your protein to get an idea if it is too dilute, too
concentrated, or somewhere in the middle. Of course, the results are
representati
l Message-
From: james09 pruza
To: CCP4BB
Sent: Thu, Jul 19, 2012 1:59 pm
Subject: [ccp4bb] Protein concentration vs Molecular wt...
Dear Crystallographers,
Is there any rule of thumb for Protein concentration and molecular
weight for crystallization trials of a soluble protein? Looking for
ther 1 mg/ml or 10mg/ml or 100mg/ml is reasonable.
Mark
-Original Message-
From: james09 pruza
To: CCP4BB
Sent: Thu, Jul 19, 2012 1:59 pm
Subject: [ccp4bb] Protein concentration vs Molecular wt...
Dear Crystallographers,
Is there any rule of thumb for Protein concentration and molec
Dear Crystallographers,
Is there any rule of thumb for Protein concentration and molecular weight
for crystallization trials of a soluble protein? Looking for high molecular
wt. protein ~50kDa.
James.
