Hi Folks,
Sorry for the non-xtallo posting.
I am curious to hear what is the longest insert anyone has cloned
using a modification of the Quikchange cloning strategy. Basically,
ligation-independent cloning by strapping on homologous regions of
the vector onto the primers which also genera
Have you seen these papers:
M Geiser, R Cebe, D Drewello, and R Schmitz. Integration of pcr
fragments at any specific site within
cloning vectors without the use of restriction enzymes and dna ligase.
Biotechniques, 31(1):88–90, 2001.
W Wang and B A Malcolm. Two-stage pcr protocol allowing introdu
I am curious to hear what is the longest insert anyone has cloned
using a modification of the Quikchange cloning strategy. Basically,
ligation-independent cloning by strapping on homologous regions of
the vector onto the primers which also generate the initial PCR
product. I plan to proceed with m
rrect
template/insert ratio.
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Raji
Edayathumangalam
Sent: Monday, December 01, 2008 3:54 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Quikchange cloning: Insert length
Hi Folks,
Sorry for the non-x
