I have few personal remarks about revere matrix seeding protocol suggested here
just an addition to Artems suggested protocol
harvesting the whole drop of interest invite guaranteed
salt crystals in the second round especially if one is
using the same screen back.
If one found crystals all over
Hm,
I wouldn't call these micro crystals anymore, I mount those things and get
datasets from them (sometimes). In my world 50 µm is defined as big.
Jürgen
On Oct 29, 2011, at 2:51 PM, Pius Padayatti wrote:
In short if you found microcrystals (15 to 20 um)
harvesting them is what i found is
Hi all,
I am trying to optimize crystals and have heard of a technique where one can
prepare a seedstock from existing crystals and use it to broadly re-screen from
scratch for hits in new conditions.
I have access to a mosquito robot and was wondering if anyone has a protocol or
I would be glad to share ours.
Artem
On Oct 28, 2011 1:29 PM, Watson, Randy ewats...@uthsc.edu wrote:
Hi all,
I am trying to optimize crystals and have heard of a technique where one
can prepare a seedstock from existing crystals and use it to broadly
re-screen from scratch for hits in new
By popular request here's my favorite version of the in-screen seeding. We
use a Mosquito but it doesn't have to be a specific robot as long as it can
dispense relatively tiny volumes of seed stock.
Caveats: (1) if I am desperate enough to do this, then the situation is
pretty bad indeed and I
