Re: [ccp4bb] Solvent-exposed Cys and an unidentified ligand

2009-06-23 Thread Lynn Gregory-Pauron
Martyn *From:* Lynn Gregory-Pauron *To:* CCP4BB@JISCMAIL.AC.UK *Sent:* Tuesday, 23 June, 2009 9:11:28 AM *Subject:* Re: [ccp4bb] Solvent-exposed Cys and an unidentified ligand Thank you for your reply. I don't actual

Re: [ccp4bb] Solvent-exposed Cys and an unidentified ligand

2009-06-23 Thread Lynn Gregory-Pauron
Thank you for your reply. I don't actually see anything interacting with what would be the second SH of the DTT. The atom opposite the Cys sulfur is at a distance of 3.0 A (could this eventually suggest hydrogen bonding)?? I have been looking at examples in the PDB, and have found a few protei

[ccp4bb] Solvent-exposed Cys and an unidentified ligand

2009-06-22 Thread Lynn Gregory-Pauron
My structure has a Cys that is solvent-exposed and an unidentified 'blob' of density which is connected to the sulfur of the Cys. The protein was crystallized in presence of DTT, and DTT does fit the density. However, I do not know if it is oxidized or not, as the density does not appear to acc