Dear All,
I am looking for a method to quickly differentiate between salt and protein
crystals. I have been told thats its a popular alternative to the
commercially available izit dye. I would appreciate if some one would share
their comassie crystal staining protocol.
Swastik
reliable. Salt crystals
react to attacks from glass fibres like rocks (as indeed what they are).
From: Swastik Phulera
Sent: Tuesday, October 15, 2013 3:59 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Staining Crystals with comassie
Dear All,
I am looking for a method to quickly differentiate
Dear All,
izit dye is a solution containing methylene blue that you could prepare
in your lab. I usually prepare a solution of 0.05%w/v of dye in water
and then I add a volume of dye solution equals to 10% of the volume of
the drop containing the crystal to test. I prefer to add the dye
solut
Dear Swastik,
izit dye is a solution containing methylene blue that you could prepare
in your lab. I usually prepare a solution of 0.05%w/v of dye in water
and then I add a volume of dye solution equals to 10% of the volume of
the drop containing the crystal to test. I prefer to add the dye
s
-
From: Danilo Belviso
Sent: Wednesday, October 16, 2013 3:53 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Staining Crystals with comassie
Dear All,
izit dye is a solution containing methylene blue that you could prepare
in your lab. I usually prepare a solution of 0.05%w/v of dye in water
a
be diluted with water or the mother liquor
> 10x-100x for the staining. Many crystals can tolerate up to 10% DMSO.
>
> Zhijie
>
>
>
> -Original Message- From: Danilo Belviso
> Sent: Wednesday, October 16, 2013 3:53 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re:
