everybody who reacted!
Herman
-Ursprüngliche Nachricht-
Von: Mickael Blaise [mailto:mickael.bla...@gmail.com]
Gesendet: Mittwoch, 15. Oktober 2014 15:16
An: Schreuder, Herman R&D/DE
Betreff: Re: [ccp4bb] Trimming of carbohydrate chains
Dear Herman
we usually trim the sugars a
Behalf Of Bärbel
Blaum
Sent: Mittwoch, 15. Oktober 2014 15:57
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Trimming of carbohydrate chains
Depending on how you produce the protein you might want to try CHO-Lec cells
which are only able to produce the high mannose core structures of N-glycans as
,
Herman
Von: Alisa Glukhova [mailto:alis...@umich.edu]
Gesendet: Mittwoch, 15. Oktober 2014 15:37
An: Schreuder, Herman R&D/DE
Cc: CCP4BB@jiscmail.ac.uk
Betreff: Re: [ccp4bb] Trimming of carbohydrate chains
Dear Herman,
You did not specify the site of glycan attachment (is it N- or O-lonked?
one: +44-1865-287564
Fax: +44-1865-287547
https://www.strubi.ox.ac.uk/research/a-radu-aricescu
Original message
Date: Wed, 15 Oct 2014 13:03:13 +
From: CCP4 bulletin board (on behalf of
herman.schreu...@sanofi.com)
Subject: [ccp4bb] Trimming of carbohydrate chains
To: CCP4BB@
Dear Herman,
You did not specify the site of glycan attachment (is it N- or O-lonked?).
I assume it is N-linked as your carbohydrate is quite large. And what
expression system are you using?
In general you can first try PNGase F, however if you are unlucky it will
only cleave in the presence of SD
t;From: CCP4 bulletin board (on behalf of
>herman.schreu...@sanofi.com)
>Subject: [ccp4bb] Trimming of carbohydrate chains
>To: CCP4BB@JISCMAIL.AC.UK
>
>Dear Bulletin Board,
>
>I am struggling with a protein domain of 15 kDa, with about 22 kDa
>carbohydrate attach
Dear Bulletin Board,
I am struggling with a protein domain of 15 kDa, with about 22 kDa carbohydrate
attached. So far, the domain did not crystallize and I suspect the carbohydrate
may hinder crystallization. Completely removing the carbohydrate results in low
expression yields and poorly solub
