Re: [ccp4bb] abnormal I/Sigma over phi rotation range

2011-07-14 Thread ccp4
This seems somewhat weird - your Rmerge values increase with more frames as explained by Tim, but I cant see why the I/SigI should increase sharply for the 180 degree set then fall off again. if you have run Scala look at the scaling plots v batch, and resolution to see if there are any weird outli

Re: [ccp4bb] abnormal I/Sigma over phi rotation range

2011-07-11 Thread Tim Gruene
Dear Vennila, my guess is that you had a larger number of images in the batch/set starting at 180 degrees (hence the slightly incereased Rmerge for that batch) and that by the end of that run your crystal had suffered from radiation damage (hence the large Rmerge for the set starting at 360degrees

Re: [ccp4bb] abnormal I/Sigma over phi rotation range

2011-07-10 Thread Jim Pflugrath
: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] abnormal I/Sigma over phi rotation range Dear CCP4BB users, In order to determine the redundancy at which the structure can be solved, I divided the master data set of my protein into five sets with phi rotations of 45,65,90, 180 and 360 degrees. I got the