Want to thanks to all for your warm suggestions. I haven't finish all the
reading suggested here. but will.
Thanks,Manqing
Date: Thu, 12 Jul 2012 13:40:39 -0700
From: anna.s.gardb...@gmail.com
Subject: Re: [ccp4bb] cryo for high salt crystal
To: CCP4BB@JISCMAIL.AC.UK
Hello, Min.Lithium su
Hello, Min.
Lithium sulfate is a cryoprotectant at 2.0 M and sometimes even less (much
lower than the concentrations needed for ammonium sulfate), so I would try
replacing your ammonium sulfate with lithium sulfate, creating a
cryoprotectant with 0.5-1.0 M KCl, 1.4-2.0 M LiSO4, at pH 7. You might n
; ofc: (315)-228-7395
> fax: (315)-228-7935
> email: rrowl...@colgate.edu
>
>
> On 7/12/2012 12:55 PM, m zhang wrote:
>> Hi Jim,
>>
>> 25% is w/v. Thanks for the information. Will check the webinar.
>>
>> Thanks,
>> Min
>>
>>
Min
From: jim.pflugr...@rigaku.com
To: mzhang...@hotmail.com; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] cryo for high salt crystal
Date: Tue, 10 Jul 2012 17:39:56 +
Sucrose, sorbitol, Splenda, trehalose, etc, but instead of 25% (is
that w/
Hi Jim,
25% is w/v. Thanks for the information. Will check the webinar.
Thanks,Min
From: jim.pflugr...@rigaku.com
To: mzhang...@hotmail.com; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] cryo for high salt crystal
Date: Tue, 10 Jul 2012 17:39:56 +
Sucrose, sorbitol, Splenda, trehalose
You can sometimes cryoprotect crystals like this with the Mitegen low viscosity
cryo oil and their micro mount loops. If that doesn't work, then you can
gradually raise the concentration of sucrose or ethylene glycol so that your
crystals freeze well. I don't think it should take anything like
Hi Min,
I agree with Christian Roth, try malonate at high concentration, 2.8 molar
is enough. I suggest to adjust the pH to about the value of your
xtallization condition.
To avoyd salt xtals formation, humidify the environment, some wet paper
around the drop can do the job!
cheers
anna
2012/7/1
A nice review of "cryosalts" is available here:
http://dx.doi.org/10.1107/S090744497587
For crystals that "don't seem to survive paratone-N", I recommend using
a microscope equipped with a polarizer. The refractive index of
paratone-N is much closer to that of protein crystals than that o
@JISCMAIL.AC.UK
Subject: [ccp4bb] cryo for high salt crystal
regaentDear All,
I am sure this question was discussed before. But I am wondering if anyone got
the same experience as I do.
I got a crystal out of condition with 1M KCl, 1.4M Ammonium sulfate at pH7. I
tried to use glycerol, ethylene
Pressure cryocooling lowers the need for cryoprotectant dramatically.
On 07/10/12 12:28, m zhang wrote:
regaentDear All,
I am sure this question was discussed before. But I am wondering if
anyone got the same experience as I do.
I got a crystal out of condition with 1M KCl, 1.4M Ammonium sulf
Hi,
I have used pure malonate (above 3M) for a similar condition. Sometimes the
cryoprotectant alone, without reservoir works quite well
Cheers
Christian
Am Dienstag 10 Juli 2012 18:28:30 schrieb m zhang:
> regaentDear All,
> I am sure this question was discussed before. But I am wondering
...@hotmail.com
Subject: [ccp4bb] cryo for high salt crystal
To: CCP4BB@JISCMAIL.AC.UK
regaentDear All,
I am sure this question was discussed before. But I am wondering if anyone got
the same experience as I do. I got a crystal out of condition with 1M KCl, 1.4M
Ammonium sulfate at pH7. I tried
regaentDear All,
I am sure this question was discussed before. But I am wondering if anyone got
the same experience as I do. I got a crystal out of condition with 1M KCl, 1.4M
Ammonium sulfate at pH7. I tried to use glycerol, ethylene glycol, 25% sucrose,
paraton-N oil, or ammonium sulfate itse
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