80% saturated lithium sulfate should have about the correct ionic strength
to match
your crystallization conditions.
The crystals need to be transfered with as little mother liquor as
possible to
avoid lithium phosphate crystallization.
Robert Kirchdoerfer suggestion is also excellent, but
Mother liquor plus 30% glycerol or 30% glucose will cryoprotect pretty
much anything, if it does not cause crystal cracking. We have had very
good general luck with 25-30% glucose, and it's easy to prepare from
your well solution or crystallization master mix. Add 150 mg of glucose
to a
Of Jerry McCully
[for-crystallizai...@hotmail.com]
Sent: Wednesday, December 15, 2010 1:13 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryoprotectant for protein crystal grown from Di-sodium
hydrogen phosphate
Dear All;
Recently we got some crystals from the condition #51 in the SaltRx
: Re: [ccp4bb] cryoprotectant for protein crystal grown from
Di-sodium hydrogen phosphate
I've had good luck cryoprotecting high salt crystal conditions with
sodium malonate (2.0-2.5M). Start with a 5M sodium malonate solution
and dilute to 40-50% with mother liquor.
good luck,
Rob
Subject: [ccp4bb] cryoprotectant for protein crystal grown from Di-sodium
hydrogen phosphate
Dear All;
Recently we got some crystals from the condition #51 in the SaltRx
crystallization kit from Hampton research.
It contains 1.5M Na2HPO4 and 0.1M Tris(pH8.5). We am going to do a test
diffraction
