Hi Folks,
We have some membrane protein crystals that are grown in 30%PEG400,
0.1M Na Citrate pH 4.5, 0.1M LiCl. The protein is purified in DDM. The
crystals are long rods and grown under room temperature in a hanging
drop set up. But once we open the cover slip, we see the rods start to
bre
You probably use hanging drops. It's the surface tension effect. Check
if sitting drops are better.
Maia
Sitting drops
weikai wrote:
Hi Folks,
We have some membrane protein crystals that are grown in 30%PEG400,
0.1M Na Citrate pH 4.5, 0.1M LiCl. The protein is purified in DDM. The
crystals
you could try sitting drops. Perhaps you can fish a crystal quicker, before it
degrades. In sitting drops the crystals may also be further away from the drop
surface and take longer to degrade.
Or quickly add mineral oil to cover the sitting drop and fish the crystals
through the oil.
If this
Weikai,
What you might be experiencing is a detergent effect, i.e., you are
near a detergent-dependent crystallization boundary. We have been hit
with this many times. Under vapor diffusion conditions, sitting or
hanging drop, the protein-detergent complex crystallizes and the free/
bound