In case you are using a "standard" purification protocol (with, say, two
purification steps and a polishing gel filtration) you might also try adding yet
another purification step, no matter how pure your sample looks on SDS-PAGE.
Tim
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Tim Gruene
Institut fuer anorganische Chemie
Tammannstr.
Have you tested how well they diffract? You should do that first. Sometimes
small, ugly looking crystals can give good data.
ho
Hi,
Have you also considered substituting the components of your initial
crystallization hit. For example, you can substitute the original PEG with
others PEGs. You can also systematically replace the cations (CaCl2, MgCl2,
etc) and anions (CaCl2, CaOAc, etc) from the original conditions. This
a
Hi,
i'm not sure if you try just seeding in your original condition. If yes, you
could try matrix seeding. Another option would be addtitves or the silver
bullets from McPherson. Sometimes sitting drop vs. hanging drop make a
difference.
Mutations at the surface are quite often an excellent alt
Hi All,
I am dealing with a protein that crystallizes in 1D. The broom stick crystals
does not yield with any improvement w.r.t their dimension. I have tried using
different concentration of salt, ppt and pH around the parent condition, even
tried seeding and temperature changing. However, any
