Re: [ccp4bb] improve protein-DNA complex crystal

2011-11-21 Thread zq deng
my DNA contains a 9-1-9 palindromic sequence,i tried different blunt end DNA,and got two kind of crystal with different lenght DNA.both don't diffract. 2011/11/21 Michael Murphy > Deng, could you tell us a bit more about the DNA that you used? I think > you may want to try to optimize the DNA

[ccp4bb] improve protein-DNA complex crystal

2011-11-20 Thread dengzq1987
Hi all, I got a protein-DNA complex crystal,by running SDS-PAGE and nativePAGE,i prove that the crystal contain both protein and DNA.BUT it does not diffract. does anyone have suggestion to improve the diffraction ability ? Best wishes, deng