[ccp4bb] linking PLP-Lys

2012-07-23 Thread Rajesh Kumar
Dear All, My friend needs a help.What is the best way to connect Lys to PLP with covalent bond.I am sure there are many ways do it. My friend would appreciate if you could simplify and explain this so that he could learn it without difficulties. Also I could learn I appreciate your time and

Re: [ccp4bb] linking PLP-Lys

2012-07-23 Thread Bernhard Rupp (Hofkristallrat a.D.)
@JISCMAIL.AC.UK Subject: [ccp4bb] linking PLP-Lys Dear All, My friend needs a help. What is the best way to connect Lys to PLP with covalent bond. I am sure there are many ways do it. My friend would appreciate if you could simplify and explain this so that he could learn it without

Re: [ccp4bb] linking PLP-Lys

2012-07-23 Thread Garib N Murshudov
Dear Rajesh tutorial on this website is designed exactly for PLP-LYS links http://www.ysbl.york.ac.uk/mxstat/JLigand/index.html regards Garib On 23 Jul 2012, at 18:30, Rajesh Kumar wrote: Dear All, My friend needs a help. What is the best way to connect Lys to PLP with covalent bond.

Re: [ccp4bb] linking PLP-Lys

2012-07-23 Thread David Cobessi
Dear Rajesh, You do not need to define a link between Lys and PLP: PLP linked to Lys is LLP. You can find examples in PDB. LLP is recognized by Phenix and Refmac. Kind regards, David On 07/23/2012 07:30 PM, Rajesh Kumar wrote: Dear All, My friend needs a help. What is the best way to connect

Re: [ccp4bb] linking PLP-Lys

2012-07-23 Thread bharath SR
Dear Rajesh, We found that LLP has a single bond between C4' and Nz which is not correct for internal aldimine form of PLP. Please use IT1 from RCSB. IT1 also has similarly linked PLP and Lys with the correct double bond. For some other PLP schiff base complexes there are multiple entries in