Dear Peter,
my guess is that isopropanol might have evaporated and, in general, that the
concentration of PEG4000
might be higher that in the starting solution because of evaporation.
I would try to decrease the isopropanol concentration, or even eliminate it.
As a cooker test if you
Hi all,
I recently got some hits from a Mosquito grown at 18C with PEG4000 and 10%
propanol. I've tried reproducing these hits using the standard 24 well format
with no success, even with playing with PEG and propanol concentrations. The
initial screening solution was from a kit that's been
Peter
We make a 96-well plate that was originally made for microbatch-under-oil,
which has a large moat or well around the whole plate.
My old friend Lesley Haire had a lot of success with this plate for a
condition that contained isopropanol.
She set up the drops without isoprop, covered them
Doesn't seem like there'd have been much isopropanol left in that tube
after 2 years. In fact, your PEG may have gone off as well, changing
the pH and god knows what else. Try screening pH and leaving out
isopropanol in your optimization.
phx
On 3 October 2012 17:37, Peter Hsu
