t, Wang Laboratory
> Harvard Medical School
> Dana-Farber Cancer Institute
> Boston, MA
> Peking University
> The College of Life Sciences
> Beijing, China
>
>
>
> ------
> Date: Thu, 19 Jul 2012 21:23:59 +0100
> From: mohamed.n...@ul.ie
&g
SciencesBeijing, China
Date: Thu, 19 Jul 2012 21:23:59 +0100
From: mohamed.n...@ul.ie
To: lfi...@hotmail.com
Subject: Re: [ccp4bb] off topic Thermal shift assay
Dear Lorenzo
> a measure of protein cooperatively,
Regard
, China
Date: Thu, 19 Jul 2012 11:00:28 -0400
From: jubo...@jhsph.edu
Subject: Re: [ccp4bb] off topic Thermal shift assay
To: CCP4BB@JISCMAIL.AC.UK
Yes that is very true. But I assume true membrane proteins exclude high
throughput :-)
However the cytoplasmic part might be fine in this case and
Yes that is very true. But I assume true membrane proteins exclude high
throughput :-)
However the cytoplasmic part might be fine in this case and then I would just
go for Sypro Orange.
Jürgen
On Jul 19, 2012, at 10:39 AM, Edwin Pozharski wrote:
My understanding is that the advantage of the
My understanding is that the advantage of the thermofluor assay is that
you can test many conditions rapidly unless of course you have some kind
of high throughput CD spectrometer in mind.
> If you have a
CD available (not the one with music on it) you don't need a
> dye
just sufficient protein
If you have a CD available (not the one with music on it) you don't need a dye
just sufficient protein and you can thermal denature your protein assuming it
contains some secondary structure elements.
Jürgen
On Jul 19, 2012, at 4:26 AM, anita p wrote:
Hi All,
I want to use a thermofluor for t
Dear Anita,
here is a link to a talk with recommended literature:
http://thermofluor.org/resources/Maksel_Protein-Stability.pdf.
Probably, you will find there the answers you need.
I recommend to try several dyes if available, due to the different
behaviour of proteins and no general rule for
Hi All,
I want to use a thermofluor for the thermal shift assay. My proteins are
cytoplasmic truncations of membrane protein. I have read about ANS,
sypro-orange and CPM. Which is the once that is popularly used by the
crystallographers for condition optimization for crystallization ??
I have read
