Re: [ccp4bb] off topic detection of oligomerisation

2012-07-11 Thread Carlos Kikuti
Urea?!?!? My arm hair went up. Anyways, DLS (Dynamic Light Scattering) might help. Hard to imagine something quicker and easier, provided that someone next to you already has a DLS machine. (sorry for the late answer) Carlos Em 26/06/2012, às 15:10, Brad Bennett escreveu: Native PAGE

[ccp4bb] off topic detection of oligomerisation

2012-06-26 Thread Careina Edgooms
Dear ccp4 bulletin board I apologise for off topic question. I wonder if anybody knows of a good method to detect oligomerisation?  I suspect an equilibrium intermediate is forming oligomers based on tryptophan fluorescence showing an exposed tryptophan becoming buried in a hydrophobic region.

Re: [ccp4bb] off topic detection of oligomerisation

2012-06-26 Thread Brad Bennett
Native PAGE (i.e. BN-PAGE), light scattering (i.e. MALLS) Not quick and easy but could work: AUC (i.e. a sedimentation equilibrium experiment) -Brad On Tue, Jun 26, 2012 at 9:01 AM, Careina Edgooms careinaedgo...@yahoo.comwrote: Dear ccp4 bulletin board I apologise for off topic question. I