Urea?!?!? My arm hair went up.
Anyways, DLS (Dynamic Light Scattering) might help. Hard to imagine something
quicker and easier, provided that someone next to you already has a DLS machine.
(sorry for the late answer)
Carlos
Em 26/06/2012, às 15:10, Brad Bennett escreveu:
Native PAGE
Dear ccp4 bulletin board
I apologise for off topic question. I wonder if anybody knows of a good method
to detect oligomerisation?
I suspect an equilibrium intermediate is forming oligomers based on tryptophan
fluorescence showing an exposed tryptophan becoming buried in a hydrophobic
region.
Native PAGE (i.e. BN-PAGE), light scattering (i.e. MALLS)
Not quick and easy but could work: AUC (i.e. a sedimentation equilibrium
experiment)
-Brad
On Tue, Jun 26, 2012 at 9:01 AM, Careina Edgooms
careinaedgo...@yahoo.comwrote:
Dear ccp4 bulletin board
I apologise for off topic question. I