Re: [ccp4bb] polarizer

2020-08-16 Thread Alexander Aleshin
Ethan, Thank you for describing existence of two types of polarizers. I use two crystal imagers/incubators, a smaller machine is easy to adjust to compensate for birefringence of plastic plates/covers, another is more capricious and the compensation is uneven over a plate. Company engineers

Re: [ccp4bb] polarizer

2020-08-16 Thread Ethan A Merritt
On Sunday, 16 August 2020 12:14:59 PDT Diana Tomchick wrote: > If only glass is placed between the polarizer and analyzer, the crystal will > not show artificial colors (try it in a 9-well Pyrex depression plate). The > artificial colors come from the diffraction of visible light from the

Re: [ccp4bb] polarizer

2020-08-16 Thread Diana Tomchick
If only glass is placed between the polarizer and analyzer, the crystal will not show artificial colors (try it in a 9-well Pyrex depression plate). The artificial colors come from the diffraction of visible light from the plastic ware, which depending upon the type of plastic and the way the

Re: [ccp4bb] polarizer

2020-08-16 Thread Nukri Sanishvili
Hi All, Adding some more details to what's been said already. Only because I've seen too many times the polarizers being used incorrectly. First, you need two polarization filters which are typically called polarizer and analyzer. First one (the polarizer) lets through only the light waves of a

Re: [ccp4bb] polarizer

2020-08-16 Thread Boniecki, Michal
AIL.AC.UK> on behalf of Matthias Zeug <mailto:matthias.z...@gmx.de> Sent: Sunday, August 16, 2020 9:05 AM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> <mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] polarizer EXTERNAL MAIL Hi all, The polarizer-microscope

Re: [ccp4bb] polarizer

2020-08-16 Thread V Nagarajan
@JISCMAIL.AC.UK *Subject:* [ccp4bb] polarizer EXTERNAL MAIL Hi all, The polarizer-microscope in our facility is not working properly, and I have to check my plates using a standard stereo-microscope. As a workaround, I thought about buying one at Amazon, placing it on top of the plates and rotating

Re: [ccp4bb] polarizer

2020-08-16 Thread Diana Tomchick
of Matthias Zeug Sent: Sunday, August 16, 2020 9:05 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] polarizer EXTERNAL MAIL Hi all, The polarizer-microscope in our facility is not working properly, and I have to check my plates using a standard stereo-microscope. As a workaround, I thought about

[ccp4bb] polarizer

2020-08-16 Thread Matthias Zeug
Hi all, The polarizer-microscope in our facility is not working properly, and I have to check my plates using a standard stereo-microscope. As a workaround, I thought about buying one at Amazon, placing it on top of the plates and rotating it to still test for birefringence. The product is