Consider also the possibility that your SeMet protein differs from the
native protein by something other than the sulfur -- selenium. Growing your
cells in different conditions may induce different host proteins that could
modify your protein. (Often we use rich media for native protein
Dear all,
Sorry for an off-topic query.
I have been unable to crystallize a Se-met containing protein (8 Met
in 206 amino acids) in the native crystallization condition ( 0.1 M
Tris pH 8.5, 1.2 M K-Na-tartrate; Theoretical pI of protein is 8.4).
As expected, solubility of Se-Met containing protein
Have you thought about phasing off the sulfurs? This is quite a common
technique nowadays.
Jim
On Tue, 27 May 2008, Joe Smith wrote:
Dear all,
Sorry for an off-topic query.
I have been unable to crystallize a Se-met containing protein (8 Met
in 206 amino acids) in the native crystallization
-seeding with S-Met crystals into Se-Met protein worked for me on
several occasions.
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Joe
Smith
Sent: Tuesday, May 27, 2008 5:11 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Problem with crystallization
of Chemistry, KU
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Jennifer Han-Chun Tsai
Sent: 13. maj 2008 18:17
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] problem of crystallization
Hi,
This topic is not related to CCP4. I am having problem of crystallizing
one protein
... your protein is the dream of any NMR spectroscopist. Small and
ultra-soluble.
Maybe just do the structure by NMR? It should be totally
straightforward for any NMR lab.
A.
On May 13, 2008, at 18:16, Jennifer Han-Chun Tsai wrote:
Hi,
This topic is not related to CCP4. I am having
Subject: Re: [ccp4bb] problem of crystallization
... your protein is the dream of any NMR spectroscopist. Small and
ultra-soluble.
Maybe just do the structure by NMR? It should be totally straightforward for
any NMR lab.
A.
On May 13, 2008, at 18:16, Jennifer Han-Chun Tsai wrote:
Hi
OX3 7BNhttp://www.oppf.ox.ac.uk **
Original message
Date: Tue, 13 May 2008 11:16:41 -0500
From: Jennifer Han-Chun Tsai [EMAIL PROTECTED]
Subject: [ccp4bb] problem of crystallization
To: CCP4BB@JISCMAIL.AC.UK
Hi,
This topic is not related to CCP4. I am
Hi,
This topic is not related to CCP4. I am having problem of crystallizing
one protein. It's a pretty small protein with size around 15kDa. I have
stock concentration around 100mg/mL. Crystallization plates I set up are
with concentration of 10mg/mL, 30mg/mL and 50mg/mL. All the plates had
Try reductive methylation of surface lysines.
http://dx.doi.org/10.1016/j.str.2006.09.005
Cheers,
Stephen
On 5/13/08, Jennifer Han-Chun Tsai [EMAIL PROTECTED] wrote:
Hi,
This topic is not related to CCP4. I am having problem of crystallizing
one protein. It's a pretty small protein
I would start looking at the buffer conditions for the protein. One place
to start is to reduce the salt. I would half, quarter, and then try no
salt at all. I
Kevin P Madauss V-161a
US Structural Biology GlaxoSmithKline
Office: 919.483.3759
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