Re: [ccp4bb] Salt or protein?

Thanks your suggestions! I tried Dials but couldn’t get any further. There just too few spots like Artem suggested. I’ll follow Patrick’s advice to seed a new screening with this crystal. This one was really tiny. I may get a bigger one. Jan > On Feb 17, 2018, at 2:43 PM, Harry Powell

Re: [ccp4bb] Salt or protein?

Hi Jan What happens if you use a program that can easily use spots from all images to index, like DIALS or XDS? My recollection (which may be wrong) is that this is not straigthforward in HKL3000. > On 17 Feb 2018, at 19:32, Jan van Agthoven wrote: > > Dear all, > At

Re: [ccp4bb] salt or protein...

Sorry Jay, They are for sure phosphate crystals. They always looked like that. psp On Sun, May 29, 2011 at 5:18 PM, Jayashankar s.jayashan...@gmail.com wrote: Dear Friends , I need to know whether phosphate can form hexagon shaped crystals. In one particular condition i have 4 different

[ccp4bb] salt or protein

Dear Jayashankar, I had several instances where salt crystals of the same component takes different morphologies. So it is very possible, as it appears that the hexagonal forms are also those of the salt components in the precipitant/buffer. You can confirm the

Re: [ccp4bb] salt or protein crystals?

I agree - looks like small molecular diffraction. Try increasing delta-phi to catch more of the lattice to confirm - I often do a 5º image or two with the detector pushed as close as possible to check for salt diffraction when screening. The lack of low res (~15-20Å) spots around the beamstop is

Re: [ccp4bb] salt or protein crystals?

Definitely small molecule crystals. You might want to push the detector closer and use better cryo solution for further confirmation. Nian On Tue, Dec 7, 2010 at 8:14 AM, xiuwen zhang congru...@gmail.com wrote: Dear Colleagues,     Currently we got several very tiny crystals. After exposuring

Re: [ccp4bb] salt or protein?

Multiple overlapping salt lattices can sometimes look like protein diffraction, as long as you're looking in only two dimensions. However, if you can find the dominant rings, you should be able to discriminate since the c-spacing of salt would nearly always be pretty small. Consider powder

Re: [ccp4bb] salt or protein?

Hi Sreeram, assuming you have plenty of those crystals, why don't you loop a few pass them through a drop of your reservoir for washing and load them on a SDS gel ? Juergen Sreeram Mahesh wrote: Hi All! I have been trying to screen for my protein crystals, from the crystals grown