Hi folks:
A colleague of mine here is doing small angle X-ray scattering at SSRL
and finding his samples are suffering significant radiation damage.
He asked me for advice as to what potential radical scavengers might
be useful. I told him to take 10 mg of ascorbic acid and to call me in
Hi Bill,
5-10% glycerol usually helps in the majority of cases. However, some
proteins require a scan of different conditions and different protein
concentrations. Like everything else, it's protein dependent.
Changing the wavelength can also make a difference. I also know some
SAXS b
Does the beamline have an attenuator? (I'm pretty sure SSRL BL4-2 has a
series of them that can be flipped on.)
cheers,
Kushol
Kushol Gupta, Ph.D.
Mathilde Krim Fellow in Basic Biomedical Research
Van Duyne Laboratory - Univ. of Pennsylvania School of Medicine
kgu...@mail.med.upenn.edu
215-573-7
we have excellent experiences with cocoamidopropylbetaine (CAPB)
especially in SAXS experiments of protein-protein and protein-rna complexes,
js
At Thu, 9 Jul 2009 17:57:36 -0700, Susan Tsutakawa wrote:
Hi Bill,
5-10% glycerol usually helps in the majority of cases. However, some
proteins req
Hi Bill,
2mM DTT usually helps.
Besides, using a large cell which moves up and down and, of course,
low temperatures also help.
Bests
2009/7/10 William Scott :
> Hi folks:
>
> A colleague of mine here is doing small angle X-ray scattering at SSRL and
> finding his samples are suffering significan
I'll second the previous glycerol posting. However, beware condensation
problems and watch the dew point when using lower temperatures.
Condensation can really screw up the signal.
Note that scavengers that work under cryogenic conditions can actually be
worse in SAXS, instead of just an electron
There was a paper a couple years ago that suggested very low
concentration of glycerol (I think 0.5%). I recently did some
systematic comparisons of different glycerol concentrations and found
that higher concentrations do seem to worsen the so-called
concentration effects. This makes intui
