Hello,
Here is our lab's work with HEK293T in bioreactor:
Pak JE, Rini JM., X-ray crystal structure determination of mammalian
glycosyltransferases., Methods Enzymol. 2006;416:30-48.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7CV2-4MCC01F-3&_user=994540&_coverDate=12%2F31%2F200
rust Centre for Human Genetics
Division of Structural Biology
Roosevelt Drive, Oxford OX3 7BN
United Kingdom
Phone: +44-1865-287564
Fax: +44-1865-287547
Original message
>Date: Tue, 23 Mar 2010 13:39:09 +0100
>From: CCP4 bulletin board (on behalf
of Jovine Luca )
>Subject: Re:
Hi Tim,
Lactococcus lactis can also produce SeMet proteins, see:
Berntsson et al. Selenomethionine incorporation in proteins expressed in
Lactococcus lactis. Protein Sci (2009) vol. 18 (5) pp. 1121-7
Cheers,
Ronnie
On Mar 23, 2010, at 14:40, Tim Gruene wrote:
> Dear all,
>
> I already receiv
Dear Tim,
Although it is not routine, it can be done in mammalian cells too! For example,
have a look at this:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf
HTH,
Luca
Luca Jovine, Ph.D.
Karolinska Inst
Dear all,
since I am currenty preparing a lecture on crystallography I am wondering about
the status quo of the production of SeMet proteins.
In 2003, if I remember correctly, it was possible to express SeMet proteins in
E.coli and insect cells.
Has this been extended to other systems, and if so,
