Dear colleagues,
I know this is not related to ccp4 but I am in need of an answer and many
of you work with cells etc.
My building had a major malfunction of electricity and the power backup did
not kick in. My -80C freezer was without power for over 24 hours until I
found out. Because it is sm
Jackie,
We grow cells routinely and freeze pellets after fermentation. In general,
proteins are fairly stable until you break cells so you are probably ok
unless your protein is very heat labile and it sat at room temperature for
hours. However as I mentioned, there is a kind of buffering from t
Hi,
My condolences. This is not a fun experience!
Your plasmids will be OK :)
Glycerol stocks may be rescued as long as after going to room temperature
they did NOT get frozen again. Re-freeze will greatly reduce viability -
but even after one re-freeze you should be able to streak the glycerol
