[ccp4bb] Job Openings - Small Molecule Crystallographer (MicroED) and Scientific Programmer

Hi everyone, My group is looking to hire two new people to further expand our small molecule MicroED capabilities here at NanoImaging Services in San Diego, CA. This is an exciting opportunity to help grow and shape our newest service division; and bring impactful crystal structures to chemists

Re: [ccp4bb] Eukaryotic protein expression

Hi Dhiraj, For transient expression in HEK293 cells in suspension, I generally use pcDNA3.1 which also uses a CMV promoter. Yields range from nothing to 50mg/l, depending entirely on what it is you're making. I would suggest that maybe the promoter is not at fault, and perhaps the product is.

Re: [ccp4bb] Eukaryotic protein expression

Hi Dhiraj, pHLsec vector for transient and its variants for lentivirus-based expression (PMIDs 17001101 and 30455477, respectively). Best wishes, Tomas On Thu, Jun 24, 2021 at 9:42 PM Srivastava, Dhiraj wrote: > > Hi all > I am trying to express my protein in HEK293 cells for

[ccp4bb] Eukaryotic protein expression

Hi all I am trying to express my protein in HEK293 cells for crystallization purpose but getting poor expression. I am using cmv promoter. Which promoter/vector people use to get good expression in eukaryotic expression system? Thank you Dhiraj

[ccp4bb] Macromolecular Crystallography - CSHL Course

Dear Colleagues, The July 16th deadline for applications to the CSHL X-ray Methods in Structural Biology Course to be held later this year, October 4 through October 19, 2021 is rapidly approaching. Please see the attached announcement, and the official course web site is here:

Re: [ccp4bb] missing covalent bond between asparagine and N-acetylglucosamine using coot carbohydrate module and phenix.refine

Dear Jiang, to draw a line or a dotted line is a question of graphics and definitions of the specific software and has nothing to do with the specific nature of a chemical bond. "link" defines in your case only an additional chemical bond that is not defined for the common polypeptide chain.

Re: [ccp4bb] missing covalent bond between asparagine and N-

Hi Jiang,I can understand why you are confused. I think I faced such case once. But Paul & Folmer's description is correct, that's the way coot works. Here is the way I solved it.In coot, after linking save the pdb without hydrogen.(Optional, if Phenix says it needs cif file - do

[ccp4bb] Last days to register: PSB Symposium "Frontiers in Bioimaging", 1-2 July 2021 - FREE registration, deadline 27 June

Hi all, Last days to register (*It's FREE.* *Deadline on 27 June 2021*) https://www.esrf.fr/psbsymposium2021 The symposium includes an exciting collection of invited speakers, selected talks, interactive virtual posters, and interactive virtual

Re: [ccp4bb] missing covalent bond between asparagine and N-acetylglucosamine using coot carbohydrate module and phenix.refine

Hello guys, I am a little bit confused. Since the polysaccharide is attached to asparagine via covalent bond. I am not sure what “link” means anyway. Does it mean some other chemical bonds? Or not chemical bond at all. As you can see from the book link below, the nitrogen atom from

[ccp4bb] E.coli RNA polymerase

Dear All, First of all, excuse me for writing an off-topic subject. Our group is working on high throughput screening of a non-coding RNA to investigate its therapeutic potential. We require *E.coli* RNA polymerase and *E.coli* sigma-70 to perform In-vitro transcription for bulk synthesis of