Re: [ccp4bb] PDB deposition of structure solved with a Robetta model

Hi Bernhard, That sounds fine. I have used the term 'RoseTTAfold/AlphaFold2 model' in that field for a few SSGCID depositions. Many times, this field does not really provide an accurate description of the model that was used: You might trim a model, use separate domains, use MoRDa that might

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Re: [ccp4bb] Baculovirus expression system

There are several but if you discount the transfection based ones (i.e. direct DNA, no virus at all) then the speed is roughly the same - about four weeks from clone to process. FlashBac works a bit faster because it avoids the use of ecoli to recombine the bacmid but the time savings is about a

Re: [ccp4bb] Baculovirus expression system

Please follow the link https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/baculovirus-expression-system On Mon, 7 Feb 2022, 21:54 Srivastava, Dhiraj, wrote: > Hi All > sorry for the question not related to crystallography. What is > the baculovirus

Re: [ccp4bb] Baculovirus expression system

Dear Dhiraj, It may be perceived as “slow”, but we (still) use the Bac-to-Bac system from ThermoFisher (previously Invitrogen). This coupled with the pFastBac, MultiBac or biGBac vector systems generally works well; some proteins/complexes may express better in High 5 cells over Sf9/Sf21.

[ccp4bb] Baculovirus expression system

Hi All sorry for the question not related to crystallography. What is the baculovirus expression system that people use these days to get good yield in less time? Thank you Dhiraj To unsubscribe from the CCP4BB

Re: [ccp4bb] PDB deposition of structure solved with a Robetta model

What do people think about depositing the Robetta model to somewhere like Zenodo (or maybe PDB-Dev) and getting an id from that? Best wishes James On 7 Feb 2022, at 13:25, Loll, Bernhard wrote: Dear all, I have recently solved a structure by molecular replacement with a search model

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[ccp4bb] PDB deposition of structure solved with a Robetta model

Dear all, I have recently solved a structure by molecular replacement with a search model calculated by the Robetta server. I am in the process to deposit the coordinates. Now, I am wondering which information I should give on the starting model. Routinely, I would provide the the PDB code,