Hi Frank,
Once we could not crystallize a monomer protein, but we knew the
structure of a homolog dimer. We made some educated guessed mutations to
dimerize our monomer target, which were based on the structure of the
dimeric homolog. Perhaps evidence or perhaps just luck? :-)
Recently there were few articles where synthetic symmetrization was used to
enhance the crystallizability. proteins used were MBP, lysozyme and GFP to name
a few. you can search for it. one of them is -
https://www.sciencedirect.com/science/article/pii/S0969212615002890
