Recently there were few articles where synthetic symmetrization was used to enhance the crystallizability. proteins used were MBP, lysozyme and GFP to name a few. you can search for it. one of them is - https://www.sciencedirect.com/science/article/pii/S0969212615002890
[https://ars.els-cdn.com/content/image/1-s2.0-S0969212615002890-fx1.jpg]<https://www.sciencedirect.com/science/article/pii/S0969212615002890> A Suite of Engineered GFP Molecules for Oligomeric Scaffolding<https://www.sciencedirect.com/science/article/pii/S0969212615002890> Applications ranging from synthetic biology to protein crystallization could be advanced by facile systems for connecting multiple proteins together i… www.sciencedirect.com Dhiraj ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Frank von Delft <frank.vonde...@cmd.ox.ac.uk> Sent: Friday, November 12, 2021 8:52 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [External] [ccp4bb] High-order oligomers vs robust crystals - references? Hello all Two decades ago, I remember (!) much talk about a reason that bacterial proteins crystallize "more easily" is that they tend to come as oligomers (dimers and up), and that this internal symmetry made them happier to crystallize. Did anybody ever publish hard evidence? Or even, is there a primary citation for the idea? Thanks Frank ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB<http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
