Re: [galaxy-dev] Deleting datasets from server

2012-04-18 Thread diana michelle magbanua
This would work for both .dat and tmp files? I was thinking about deleting them manually but the idea of them being connected to a database was bothering me. Other than minor errors, will there be no other serious repercussions? Thanks for the reply! DM On Thu, Apr 19, 2012 at 1:34 PM, JIE CHEN

Re: [galaxy-dev] Deleting datasets from server

2012-04-18 Thread JIE CHEN
Of course. I deleted lots of times. But be careful if some of the files are important. Once you deleted them, all the files will disappear from the user's history(or when you open them, you will run into errors). You should know the files you are deleting. On Wed, Apr 18, 2012 at 8:40 PM, diana mi

[galaxy-dev] Deleting datasets from server

2012-04-18 Thread diana michelle magbanua
Hi there, I would just like to ask if I could just directly delete the .dat files from the database folder of Galaxy (or even just the tmp files). I am not allowed to delete any other file from the computer other than the ones in galaxy-dist. I have tried running the cleanup scripts, but since the

[galaxy-dev] Cuffdiff output

2012-04-18 Thread Xu, Jianpeng
Hi, I have ran Cuffdiff and got output files: transcript differential expression testing, gene differential expression testing. Could you please tell me how to get the significant differential expression gene and transcript ? Thanks, This e-mail message (inc

Re: [galaxy-dev] Private key file format issue (for connecting EC2 instance with EBS volume)

2012-04-18 Thread Dannon Baker
Mo, I just tested one of my amazon keys with PuTTYgen and the default settings and it worked. Can you verify the contents of the .pem file you're using for me? On windows I'd open it in http://notepad-plus-plus.org/ or a similar utility to see it correctly. The file should look something l

Re: [galaxy-dev] Mira-Assembler: DOESN'T WORK ON GALAXY

2012-04-18 Thread JIE CHEN
The version I installed is : mira_3.4.0_prod_linux-gnu_x86_64_static On Wed, Apr 18, 2012 at 3:31 PM, Peter Cock wrote: > On Wed, Apr 18, 2012 at 11:21 PM, JIE CHEN > wrote: > > Dear all, > > > > I just installed Mira-Assembler via Galaxy tool sheds. As expected, it > > downloaded the wrappers

Re: [galaxy-dev] Moving data about the EC2/EBS/S3 spaces on the Amazon cloud.

2012-04-18 Thread Enis Afgan
Hi Mo, On Wed, Apr 18, 2012 at 4:53 AM, Mohammad Heydarian wrote: > Hello, > I am new to cloud computing and am trying to use the Galaxy cloudman > service through Amazon to analyse NGS data. I have a couple of questions > regarding data transfer: > > If I am running an EC2 instance, with an EBS

Re: [galaxy-dev] Mira-Assembler: DOESN'T WORK ON GALAXY

2012-04-18 Thread Peter Cock
On Wed, Apr 18, 2012 at 11:21 PM, JIE CHEN wrote: > Dear all, > > I just installed Mira-Assembler via Galaxy tool sheds. As expected, it > downloaded the wrappers into the "shed-tools" directory. Then I downloaded > and installed Mira binaries on the system and add it to $PATH. Right now, > mira c

[galaxy-dev] Mira-Assembler: DOESN'T WORK ON GALAXY

2012-04-18 Thread JIE CHEN
Dear all, I just installed Mira-Assembler via Galaxy tool sheds. As expected, it downloaded the wrappers into the "shed-tools" directory. Then I downloaded and installed Mira binaries on the system and add it to $PATH. Right now, mira can run successfully in the command line prompt. However when i

[galaxy-dev] Output files from Galaxy

2012-04-18 Thread Jennifer Jackson
I have installed local galaxy and am using it to run some programs. The output files in the history seems to be stored in the directory .../galaxy-dist/database/files/000/, there are files dataset_100.dat, dataset_120.dat etc. My question is that: If I run a program, is there a way to check which

[galaxy-dev] Private key file format issue (for connecting EC2 instance with EBS volume)

2012-04-18 Thread Mohammad Heydarian
Hello, I am trying to connect an EC2 instance to an EBS volume. I have attached the EBS volume to the EC2 instance (running Galaxy). I now need to mount the EBS volume to the EC2 instance. To do this I will use Putty to make the connection. But first I have to convert the private key from ".pem" f

Re: [galaxy-dev] Merging BLAST database support into Galaxy?

2012-04-18 Thread Peter Cock
On Wed, Apr 18, 2012 at 6:10 PM, Edward Kirton wrote: > sounds great, thanks peter.  i granted you access to my toolshed repo, but > perhaps we want only one tool in the toolshed when all done. Thanks - but given the tools currently live in the main repository with Galaxy itself, I'll be focussin

Re: [galaxy-dev] Merging BLAST database support into Galaxy?

2012-04-18 Thread Edward Kirton
sounds great, thanks peter. i granted you access to my toolshed repo, but perhaps we want only one tool in the toolshed when all done. On Wed, Apr 18, 2012 at 3:20 AM, Peter Cock wrote: > On Wed, Apr 18, 2012 at 10:53 AM, Peter Cock > wrote: > > Hi Edward, > > > > We're now running BLAST+ searc

Re: [galaxy-dev] Potential database corruption with local galaxy instance

2012-04-18 Thread Jeremy Goecks
>> % sh manage_db.sh downgrade 92 >> % sh manage_db.sh upgrade > The downgrade to 92 and upgrade created job.params. This is progress. You should be able to run jobs again, yes? > Unfortunately, we are still getting errors about duplicate key values. The > debug output when I try to export a h

Re: [galaxy-dev] Displaying genomic sequences in Trackster

2012-04-18 Thread Jeremy Goecks
Hi Naharajan, My best guess is that you've found some bugs. Fortunately, they are likely fixed in galaxy-central and will soon be available in galaxy-dist (we're planning an update in the next couple days). Once galaxy-dist is updated, please try updating your Galaxy instance and seeing if that

Re: [galaxy-dev] Exception: Error Setting BAM Metadata: /bin/sh: samtools: not found

2012-04-18 Thread zhengqiu cai
Hi Ross, I have a cloudman version of Galaxy working well. What I am doing now is trying to install Galaxy on starcluster, which is a SGE cluster. I should followed the install method for the normal cluster/SGE install, not for the cloud. Thank you for your quick reply. Have a nice day. Cai --

Re: [galaxy-dev] Exception: Error Setting BAM Metadata: /bin/sh: samtools: not found

2012-04-18 Thread Ross
Hi, Cai. Apologies but I had not noticed any reference to the cloud distribution before - that changes everything - I was talking about a normal cluster/SGE install - you should definitely not need to do anything at all to the AMI - maybe someone with cloudman experience can be more helpful than I

Re: [galaxy-dev] Exception: Error Setting BAM Metadata: /bin/sh: samtools: not found

2012-04-18 Thread zhengqiu cai
Hi Ross, I did not compile samtools using the --prefix. The samtools I used comes with Galaxy AMI image, and I copied it to the same path on my EC2. How to compile samtools using --prefix? I just typed 'make', and there is not a configuration file. In the cloudman version of Galaxy (it is worki

Re: [galaxy-dev] "Fetch taxonomic representation" DOESN'T WORK

2012-04-18 Thread zhengqiu cai
--- 12年4月18日,周三, zhengqiu cai 写道: 发件人: zhengqiu cai 主题: Re: [galaxy-dev] "Fetch taxonomic representation" DOESN'T WORK 收件人: galaxy-dev@lists.bx.psu.edu, "JIE CHEN" 日期: 2012年4月18日,周三,下午10:58 --- 12年4月18日,周三, JIE CHEN 写道: 发件人: JIE CHEN 主题: Re: [galaxy-dev] "Fetch taxonomic represen

Re: [galaxy-dev] Exception: Error Setting BAM Metadata: /bin/sh: samtools: not found

2012-04-18 Thread Ross
Hi, Cai - sorry to hear it's still not working right. You've compiled/installed samtools using the --prefix configure option and tested that all the samtools based Galaxy wrappers work if you use the local job runner? That means that both the appropriate lib and bin directories are on the Galaxy u

Re: [galaxy-dev] "Fetch taxonomic representation" DOESN'T WORK

2012-04-18 Thread zhengqiu cai
--- 12年4月18日,周三, JIE CHEN 写道: 发件人: JIE CHEN 主题: Re: [galaxy-dev] "Fetch taxonomic representation" DOESN'T WORK 收件人: galaxy-dev@lists.bx.psu.edu 日期: 2012年4月18日,周三,上午5:36 On Tue, Apr 17, 2012 at 1:00 PM, JIE CHEN wrote: Thank you Nate. Your advise is very helpful to me. I installed the b

Re: [galaxy-dev] Exception: Error Setting BAM Metadata: /bin/sh: samtools: not found

2012-04-18 Thread zhengqiu cai
Hi Ross, Thank you for your reply. It still did not work. I think SGE knows the path of samtools. Here is a test case I added to Galaxy, and it creates the index for fasta file. I pasted the samtools faidx wrapper and xml files below: ubuntu@master:/mnt/galaxyTools/galaxy-dist/tools/samtools$ mo

Re: [galaxy-dev] database engine pool size

2012-04-18 Thread Dannon Baker
On Mar 26, 2012, at 12:18 PM, Shantanu Pavgi wrote: > {{{ > TimeoutError: QueuePool limit of size 40 overflow 50 reached, connection > timed out, timeout 30 > }}} > > These limits are not reached for regular (non-workflow) galaxy jobs. Any help > on optimum values for these settings or performan

[galaxy-dev] Displaying genomic sequences in Trackster

2012-04-18 Thread Naharajan Lakshmanaperumal
Dear all, We have our own galaxy instance and the idea is to have trackster enabled for users to be able to visualize NGS mapping. We were able to configure trackster in our instance and the visualization works fine. We have two questions regarding trackster: 1) We can't display genomic sequen

Re: [galaxy-dev] Hidden output files

2012-04-18 Thread Dannon Baker
On Apr 18, 2012, at 8:44 AM, Frank Sørensen wrote: > - Should I pull from galaxy-central to get this update (7057:08fbfeaaf3e1)? > - If so, could you please tell me how I do that? Sure, you can pull from galaxy-central by issuing a manual pull with source from your galaxy-dist directory: > hg p

Re: [galaxy-dev] Hidden output files

2012-04-18 Thread Frank Sørensen
Thanks. I have set up Mercurial to fetch updates from galaxy-dist, and it seems that my version of galaxy is up to date. - Should I pull from galaxy-central to get this update (7057:08fbfeaaf3e1)? - If so, could you please tell me how I do that? - Do you know how I permanently change repositor

Re: [galaxy-dev] How to specify RUM output dir?

2012-04-18 Thread Jeremy Goecks
> > I’m good with everything except the . I can’t figure out how to > pass that directory information from Galaxy to the RUM_runner.pl in such a > way that Galaxy will be able to subsequently capture the data. Does anybody > have any advice, or can someone point me towards an existing wrapper t

Re: [galaxy-dev] Exporting worflow to local instal

2012-04-18 Thread Dannon Baker
Hi Danny, For moving workflows from one instance to another you'll want to click on "Download or Export" in the workflow context menu and use the "URL for Importing to Another Galaxy". It looks something like this: (note the for_direct_import, that's how you'll know you have the right link ty

Re: [galaxy-dev] How do you connect existing EBS volumes to a fresh EC2 instance?

2012-04-18 Thread Dannon Baker
Galaxy Cloudman doesn't support automatic addition of EBS volumes yet, though it is something we're looking at long term. If the volume is already in the same Availability Zone as your cloud instance, the following steps should work: • With the volume checked, click More... -> Attach Vo

Re: [galaxy-dev] Hidden output files

2012-04-18 Thread Dannon Baker
Hi Frank, This should be resolved as of changeset 7057:08fbfeaaf3e1. Thanks! -Dannon On Mar 27, 2012, at 4:37 AM, Frank Sørensen wrote: > Hi Dannon, > > Unfortunately our server is (still) behind a firewall, so I can't share > anything, but I hope you can reproduce the error from the follow

[galaxy-dev] Tool development: how to pass parameters stored in an input dataset

2012-04-18 Thread Joachim Jacob
Hi, Is it possible to pass the parameters of an input dataset? For example: the user selects a dataset of the history. I want to pass to my wrapperscript the input parameters of that dataset (the information you see when clicking the small 'i' on a dataset in the history). Regards, Joachim

[galaxy-dev] Conditional tool options based on input file format?

2012-04-18 Thread Peter Cock
Dear all, I've looked over the wiki and as far as I can see, 'when' tags used inside 'conditional' tags only work on another input variable's values. I would like to be able to do is a conditional based on the file format of an input file. For instance, a tool might take XML or tabular files, and

Re: [galaxy-dev] [galaxy-user] Toolshed initial upload errors out

2012-04-18 Thread Greg Von Kuster
Hello Paul, I apologize for somehow missing the first line of your paster log, which in fact shows the upload step. Since you've tried uploading various files with no success, the problem is likely to be caused by something specific to your environment - possibly the version of the mercurial p

Re: [galaxy-dev] Merging BLAST database support into Galaxy?

2012-04-18 Thread Peter Cock
On Wed, Apr 18, 2012 at 10:53 AM, Peter Cock wrote: > Hi Edward, > > We're now running BLAST+ searches on our local Galaxy via our cluster, > and some of the cluster nodes have relatively small amounts of RAM. > This means I've become more aware of limitations in the NCBI BLAST+ > tools' support f

[galaxy-dev] Merging BLAST database support into Galaxy?

2012-04-18 Thread Peter Cock
Hi Edward, We're now running BLAST+ searches on our local Galaxy via our cluster, and some of the cluster nodes have relatively small amounts of RAM. This means I've become more aware of limitations in the NCBI BLAST+ tools' support for using a subject FASTA file (instead of making a local BLAST d

Re: [galaxy-dev] Tool syntax: How to get history name?

2012-04-18 Thread Louise-Amélie Schmitt
Ok, after a lot of testing and searching in the libs, I found the solution: $inputdataset.dataset.history.name Le 18/04/2012 10:22, Louise-Amélie Schmitt a écrit : Hi everyone, I as wondering if there was a way to get the history name of an input dataset within a tool (i.e. in the cheetah be

[galaxy-dev] Tool syntax: How to get history name?

2012-04-18 Thread Louise-Amélie Schmitt
Hi everyone, I as wondering if there was a way to get the history name of an input dataset within a tool (i.e. in the cheetah between the tags), is there? Thanks, L-A ___ Please keep all replies on the list by using "reply all" in your

Re: [galaxy-dev] [galaxy-user] Toolshed initial upload errors out

2012-04-18 Thread Paul-Michael Agapow
Picking up on this thread, I said: > As per the title, I'm trying to setup a toolshed for our local use. After > creating a repo, I try to upload the initial file only to get: > > TypeError: array item must be char > > Which seems to happen repeatedly, i.e. it's not a passing error. And