Re: [galaxy-dev] Galaxy - Cloudman

2014-04-25 Thread Alessia Deglincerti
Hey Dannon, I am using Galaxy (Cloudman) to analyze RNA seq data. I have a bunch of datasets that I ran TopHat on. I am now trying to run Cufflinks etc. I used the same data and workflow yesterday to run Cufflinks etc and that worked fine. I used my own reference annotation (Homo sapiens hg38)

Re: [galaxy-dev] Galaxy/Cloudman Compute Nodes User

2014-04-25 Thread Jim McCusker
Actually, both of these help. I'm putting a boto.conf file into /mnt/galaxy/galaxy-app and setting the BOTO_PATH environment variable from the python script that needs it, so it will work in dev (non-clusterman setup) and production. Jim On Fri, Apr 25, 2014 at 11:20 AM, Dannon Baker wrote: > A

Re: [galaxy-dev] Galaxy/Cloudman Compute Nodes User

2014-04-25 Thread Dannon Baker
Ahh, sorry about that, I misread the bit about it actually needing to be in the user's directory. You could use a worker_post_start_script to configure things like this (creating/copying .boto at boot from the shared space in the script). See https://wiki.galaxyproject.org/CloudMan/UserDatafor ho

Re: [galaxy-dev] Galaxy/Cloudman Compute Nodes User

2014-04-25 Thread Dannon Baker
Hi Jim, /mnt/galaxy is shared via NFS with all cluster workers -- this (or a subdirectory) should work for you. -Dannon On Fri, Apr 25, 2014 at 10:56 AM, Jim McCusker wrote: > Hi all, > > I need to add a .boto file to the user that galaxy jobs get exec'ed as on > cloudman nodes. I thought it w

[galaxy-dev] Galaxy/Cloudman Compute Nodes User

2014-04-25 Thread Jim McCusker
Hi all, I need to add a .boto file to the user that galaxy jobs get exec'ed as on cloudman nodes. I thought it would copy over what the galaxy server has in its home directory, but that doesn't seem to happen. How can I get things like my .boto file to migrate over to new cloudman nodes when they

Re: [galaxy-dev] Galaxy - Cloudman

2014-04-25 Thread Dannon Baker
Do you have Cloudman admin access on this instance? The first thing I'd check would be http:///cloud to verify that your instance has free disk space and to look for any errors in the log. Then, check out the admin service list (http:///cloud/admin) to see if there are any service issues. Next t

Re: [galaxy-dev] Galaxy - Cloudman

2014-04-24 Thread Dannon Baker
Hey Alessia, I'd love to help, but I need a little more context here. Where are you seeing this error, what exactly are you doing when it appears, and can you tell me a little bit more about the Galaxy configuration you're working with? -Dannon On Thu, Apr 24, 2014 at 8:37 PM, Jennifer Jackson

[galaxy-dev] Galaxy - Cloudman

2014-04-24 Thread Jennifer Jackson
Posting to the galaxy-...@bx.psu.edu mailing list. https://wiki.galaxyproject.org/MailingLists My instance of Galaxy seems to be stuck (same place since last night) and I have received the error message several times now. Pasting the error message below: *Details* user username

Re: [galaxy-dev] Galaxy CloudMan release - Tophat does not work

2013-08-27 Thread Enis Afgan
Tophat works for you, even after doing this it fails to >> run properly for me. However, at least now Galaxy can find the programs. >> >> >> From: Mohammad Heydarian >> Date: Fri, 12 Jul 2013 14:21:23 -0400 >> To: Microsoft Office User >> Cc: Enis Afgan ,

Re: [galaxy-dev] Galaxy CloudMan release - Tophat does not work

2013-07-13 Thread Enis Afgan
wever, at least now Galaxy can find the programs. > > > From: Mohammad Heydarian > Date: Fri, 12 Jul 2013 14:21:23 -0400 > To: Microsoft Office User > Cc: Enis Afgan , Galaxy-dev > Subject: Re: [galaxy-dev] Galaxy CloudMan release - Tophat does not work > > Cufflink

Re: [galaxy-dev] Galaxy CloudMan release - Tophat does not work

2013-07-12 Thread Ravpreet Setia
y-dev mailto:galaxy-...@bx.psu.edu>> Subject: Re: [galaxy-dev] Galaxy CloudMan release - Tophat does not work Cufflinks, Cuffcompare, and Cuffdiff are also failing to run because they can not be found. I am using the newly released version of Cloudman 2.0 (ami-118bfc78). Any help would

Re: [galaxy-dev] Galaxy CloudMan release - Tophat does not work

2013-07-12 Thread Mohammad Heydarian
e default symlink is used by Galaxy: > http://wiki.galaxyproject.org/Admin/Config/Tool%20Dependencies > > Thanks for any suggestions. > > > > From: Enis Afgan > Date: Sun, 30 Jun 2013 07:37:46 +0200 > To: Galaxy-dev > Subject: [galaxy-dev] Galaxy CloudMan release > > *Last

[galaxy-dev] Galaxy CloudMan release

2013-06-30 Thread Enis Afgan
*Last night, we released an update to Galaxy CloudMan.* CloudMan offers an easy way to get a personal and completely functional instance of Galaxy in the cloud in just a few minutes, without any manual configuration. *IMPORTANT - please read* Any new cluster will automatically start using this ver

[galaxy-dev] Galaxy, CloudMan & nginx 1.4.0

2013-04-28 Thread John Chilton
I wanted to update CloudMan to use nginx 1.4.0 for web socket proxy support, but I hit a problem. nginx-upload-module does not work with nginx 1.4.0 and the author has no intention of continuing work on the module. https://github.com/vkholodkov/nginx-upload-module/issues/41 There is a patch that

Re: [galaxy-dev] galaxy/cloudman failure handling

2012-11-12 Thread Dannon Baker
On Nov 12, 2012, at 10:23 AM, Jorrit Boekel wrote: > I was therefore looking for fault tolerance mechanisms in the galaxy project, > which I seem to remember existed. Somehow I can't find anything about it > right now though. > > I've tested a little bit, and it seems that as soon as one reboo

[galaxy-dev] galaxy/cloudman failure handling

2012-11-12 Thread Jorrit Boekel
Dear list, I would like to start using Amazon's spot pricing model for my Galaxy/Cloudman instances (e.g. an on demand master node with spot instance worker nodes). However, this means that Amazon at times of spot prices higher than my set price limit will shutdown my instances without notion. I

[galaxy-dev] galaxy cloudMan failed

2012-07-26 Thread jiesh...@bioteam.net
Hi, all I just tried installing galaxy in amazon cloud following the instruction here:http://wiki.g2.bx.psu.edu/CloudMan. Using this ami-da58aab3, I do not have http service after the instance is started. I logined into the instance and confirmed it. How should I troubleshoot it? Could your g

Re: [galaxy-dev] Galaxy Cloudman - How to analyse > 1TB data ?

2012-02-21 Thread Wetzels, Yves [JRDBE Extern]
running multiple Galaxy Cloudman instances to circumvent the 1 TB limit. If I find some time I will redo the exercise with the LVM. Kind Regards Yves From: Enis Afgan [mailto:eaf...@emory.edu] Sent: Sunday, 19 February 2012 23:50 To: Wetzels, Yves [JRDBE Extern] Cc: galaxy-dev@lists.

Re: [galaxy-dev] Galaxy Cloudman - How to analyse > 1TB data ?

2012-02-19 Thread Enis Afgan
Hi Yves, When you create the LVM file system - are you composing it from the volume that already contains the data (i.e., directory structure++ created by CloudMan) and then adding another volume into the LVM or starting with 2 new, clean volumes? Maybe trying again and not messing with SGE at all

Re: [galaxy-dev] Galaxy Cloudman - How to analyse > 1TB data ?

2012-02-16 Thread Wetzels, Yves [JRDBE Extern]
Hi Brad I did not restart the master CloudMan node. I only restarted the services (Galaxy, PostgreSQL and SGE). I do not have these problems without creating the logical volume. Kind Regards Yves Yves; I'm hoping Enis can jump in here since he is more familiar with the internals of CloudMan an

Re: [galaxy-dev] Galaxy Cloudman - How to analyse > 1TB data ?

2012-02-15 Thread Brad Chapman
Yves; I'm hoping Enis can jump in here since he is more familiar with the internals of CloudMan and may be able to offer better advice. I can tell you what I see from your error messages. > I used LVM2 to create the logical volume. Does this involve stopping and restarting the master CloudMan no

Re: [galaxy-dev] Galaxy Cloudman - How to analyse > 1TB data ?

2012-02-14 Thread Brad Chapman
Yves; > I am currently investigating if Galaxy Cloudman can help us in analyzing > large NGS datasets. > > I was first impressed by the simple setup, the autoscaling and > useability of Galaxy Cloudman but soon ran into the EBS limit of 1 TB L > > I thought to be clever and umounted the /mnt/ga