Re: [galaxy-user] GATK best practices with local installation of Galaxy

2011-08-03 Thread Camille Stephan
Hi Ross, thanks for your answer. I found a dirty fix for merging pairs of bam files, had to change a couple of things in my local installation though. - Add group reads to each BAM file separately using Picard's Add or Replace Groups http://localhost:8080/tool_runner?tool_id=picard_ARRG (with

Re: [galaxy-user] (no subject)

2011-08-03 Thread Jennifer Jackson
Hello Tarek, If you want to reduce the number of identical reads, then please see the tool FASTA manipulation - Collapse sequences. Converting formats would be necessary, the tool Tabular-to-FASTA can perform this operation. Best, Jen Galaxy team On 8/2/11 3:57 PM, tarek addwebi wrote:

Re: [galaxy-user] visualization

2011-08-03 Thread vasu punj
IGV should allow you to do this  but not sure about trackbrowser in Galaxy. Vasu --- On Wed, 8/3/11, Jiannong Xu j...@nmsu.edu wrote: From: Jiannong Xu j...@nmsu.edu Subject: [galaxy-user] visualization To: galaxy-user@lists.bx.psu.edu galaxy-user@lists.bx.psu.edu Date: Wednesday, August 3,

Re: [galaxy-user] GATK best practices with local installation of Galaxy

2011-08-03 Thread Ross
Hi, Camille, I can see this really needs a 'proper' fix - preferably taking advantage of the automated header merge. Preserving the metadata from each bam automatically is safer and less error-prone but you could use the existing Replace sam/bam header tool to do the surgery once you have a

Re: [galaxy-user] GATK best practices with local installation of Galaxy

2011-08-03 Thread Ross
Hi, Camille. If you can find some time to upload some of your bam files, could you please test the revised bam merge tool on http://test.g2.bx.psu.edu/ and let me know how you go. This won't be on the main site until the next scheduled update in a few weeks. If you need this locally, the changes