Re: [galaxy-user] Blast2GO local instance Re: Table with gene count reads

2012-04-03 Thread Peter Cock
On Mon, Apr 2, 2012 at 6:41 PM, Greg Von Kuster wrote: > > On Mar 24, 2012, at 7:30 AM, Peter Cock wrote: >> >> Have you seen the README file that comes with the >> Blast2GO wrapper? Perhaps the 'install from toolshed' >> could be tweaked to make this kind of documentation >> more visible... > > I

Re: [galaxy-user] Blast2GO local instance Re: Table with gene count reads

2012-04-03 Thread Greg Von Kuster
On Apr 3, 2012, at 6:07 AM, Peter Cock wrote: > On Mon, Apr 2, 2012 at 6:41 PM, Greg Von Kuster wrote: >> >> On Mar 24, 2012, at 7:30 AM, Peter Cock wrote: >>> >>> Have you seen the README file that comes with the >>> Blast2GO wrapper? Perhaps the 'install from toolshed' >>> could be tweaked t

Re: [galaxy-user] Cloudman - Problem starting shared cluster

2012-04-03 Thread mailing list
How do I know which snapshot is the appropriate one? The folder Dannon mentioned seems to be missing. I swear this sharestring worked when I tested it two months ago. I can't imagine what happened. -Greg On Mon, Apr 2, 2012 at 6:48 PM, Enis Afgan wrote: > Along with the folder structure Danno

[galaxy-user] input for GATK DepthOfCoverage

2012-04-03 Thread Lilach Friedman
Hi Galaxy Team, I'm trying to use GATK DepthOfCoverage, and to give a BAM file of alined results as input. My steps were: 1. BWA alignment 2. SAM-to-BAM 3. rmdup. when I'm trying to give 3 (rmdup results) as an input to GATK DepthOfCoverage, an error appears: " Sequences are not currently avai

Re: [galaxy-user] Cloudman - Problem starting shared cluster

2012-04-03 Thread Brad Chapman
Greg; > How do I know which snapshot is the appropriate one? The Amazon console displays the description for snapshots. They should have a string like: CloudMan share-a-cluster cluster_name: cm-31bf91279e41769ff78af22c1a276ec > The folder Dannon mentioned seems to be missing. > > I swear this

Re: [galaxy-user] Cloudman - Problem starting shared cluster

2012-04-03 Thread mailing list
Thanks Brad. Your last point sounds suspiciously like something I would do ... So whenever I share I can terminate my instance but I shouldn't remove any data? Is that correct? Thanks, Greg On Tue, Apr 3, 2012 at 9:18 AM, Brad Chapman wrote: > > Greg; > >> How do I know which snapshot is the

Re: [galaxy-user] input for GATK DepthOfCoverage

2012-04-03 Thread Lilach Friedman
I fixed it, Thanks. It was because I aligned to different genomes in BWA and GATK steps 2012/4/3 Lilach Friedman > Hi Galaxy Team, > I'm trying to use GATK DepthOfCoverage, and to give a BAM file of alined > results as input. > > > My steps were: > 1. BWA alignment > 2. SAM-to-BAM > 3. rmdup. >

Re: [galaxy-user] Cloudman - Problem starting shared cluster

2012-04-03 Thread Brad Chapman
Greg; > Thanks Brad. Your last point sounds suspiciously like something I would do ... > > So whenever I share I can terminate my instance but I shouldn't remove > any data? Is that correct? What I do is keep multiple instances with different cluster_names: - A stable instance to store shared

[galaxy-user] Annotation

2012-04-03 Thread hsharm03
Dear galaxy users, This might be a very basic question to most of you. But I was hopimg I could get better understanding of this concept by asking you all. How exactly can we accomplish annotation of our reads? The combination of Tophat and cufflinks does annotate genes right? . I am a bit confus

Re: [galaxy-user] Disk space quota Issue

2012-04-03 Thread Jennifer Jackson
Hello Ateeq, You are correct, the disk space for accounts is a fixed amount on the public main Galaxy instance. Permanently deleting (sometimes called "Purge/purging" in the UI) datasets and histories is the method to use to create more working space. http://wiki.g2.bx.psu.edu/Main#User_data

Re: [galaxy-user] Galaxy Server running error in fetching sequences

2012-04-03 Thread Anton Nekrutenko
Hong: I'm forwarding this to our official user mailing list. Please, use it in the future for your inquiries. Thanks! anton Anton Nekrutenko http://nekrut.bx.psu.edu http://usegalaxy.org On Apr 3, 2012, at 4:37 PM, xu hong wrote: > Hi Anton, > > I'm a biological student using Galaxy sev

[galaxy-user] FASTQ joiner fails to join PE data.

2012-04-03 Thread meganathan pr
Hi, I have HiSeq2000 paired end sequence data in two separate FASTQ files. I need to filter the low quality scored sequences from my data to have a good assembly. So I decided to join the PE reads and then filter the low quality sequences in Galaxy. To do this first I groomed the data using