[galaxy-user] Do I need to have tool or dependencies installed in the server to run Galaxy tools?

2012-10-17 Thread Sachit Adhikari
I integrated Bismark in Galaxy in my server. I downloaded Bismark from Galaxy Tool shed. I just copied the Bismark into /tools directory and added some codes in tool_conf.xml to make it visible. Do I need to install the real Bismark application in the server too? I am really confused here. Thanks _

Re: [galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Sachit Adhikari
Thanks. Helped me! On Thu, Oct 18, 2012 at 11:02 AM, Enis Afgan wrote: > With a certain version of Python there's been an issue stopping Galaxy > using ctrl+C. You can use 'sh run.sh --daemon' to have the process run in > the background and then 'sh run.sh --stop-damemon' to stop it. > > Hope th

Re: [galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Enis Afgan
With a certain version of Python there's been an issue stopping Galaxy using ctrl+C. You can use 'sh run.sh --daemon' to have the process run in the background and then 'sh run.sh --stop-damemon' to stop it. Hope this helps, Enis On Thu, Oct 18, 2012 at 4:12 PM, Sachit Adhikari < sachit.techner..

[galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Sachit Adhikari
Hello Everyone. Ctrl+C or Ctrl+D doesn't stop the Galaxy server. In local machine, I need to close the terminal and restart the terminal again. However, in server I integrated several tools, now I need to restart the server to test it. I used ./run.sh --reload doesn't restart the server and I can't

Re: [galaxy-user] Export to file

2012-10-17 Thread Jeremy Goecks
Dave, There's likely something problematic about your history that causing problems. Can you share with me the history that's generating the error? To do so, from the history options menu --> Share/Publish --> Share with a User --> my email address Thanks, J. On Oct 17, 2012, at 6:58 PM, Jen

Re: [galaxy-user] Export to file

2012-10-17 Thread Jennifer Jackson
Hi Dave, Yes, if your Galaxy instance is on the internet, for entire history transfer, you can skip the curl download and just enter the URL from the public Main Galaxy server into your Galaxy directly. To load large data over 2G that is local (datasets, not history archives), you can use th

Re: [galaxy-user] cuffcompare input

2012-10-17 Thread Jennifer Jackson
Hello, Yes, this has not changed. The reference GTF given to Cuffdiff must contain all of the transcripts of the inputs: http://user.list.galaxyproject.org/cuffcompare-inputs-td4654384.html Run this as you have done previously: http://user.list.galaxyproject.org/cuffcompare-outputs-missing-td4

Re: [galaxy-user] Export to file

2012-10-17 Thread Jennifer Jackson
Hi Dave, To export larger files, you can use a different method. Open up a terminal window on your computer and type in at the prompt ($): $ curl -0 '' > name_the_output Where can be obtained by right-clicking on the disc icon for the dataset and selecting "Copy link location". If you are

[galaxy-user] Export to file

2012-10-17 Thread Dave Corney
Hi list, Is there a currently a known problem with the "export to file" function? I'm trying to migrate some data from the public galaxy to a private one; the export function worked well with a small (~100mb) dataset, but it has not been working with larger datasets (>2GB) and I get the error: Ser

Re: [galaxy-user] cuffdiff values different for same sample

2012-10-17 Thread Jennifer Jackson
Hello, Thank you for sharing your history. The difference in FPKM values can be explained by the use of the -N option ("Perform quartile normalization: Yes"). Set this to "No" to avoid the variable per-run normalization. This has also been discussed at seqanswers.com: http://seqanswers.com/fo

Re: [galaxy-user] The last column in the output file of 'Screen Motif'

2012-10-17 Thread Jennifer Jackson
Hello, This tool is hosted on the Cistrome Analysis Pipeline (a custom Galaxy public server). It would be best to contact them about the tool to be certain that you receive the most current information. Their support email (from the login page) is: cistrome-b...@jimmy.harvard.edu. http://wik

Re: [galaxy-user] Error installing MACS on local Galaxy

2012-10-17 Thread Jennifer Jackson
Hello Jerzy, The MACS wrapper in Galaxy currently supports version 1.3, which explains the problems with 1.4. Tool dependencies are noted on this wiki: http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Dependencies For the other errors, are you trying to run MACS on the command line or within Gala

Re: [galaxy-user] FW: problem in data uploading

2012-10-17 Thread Jennifer Jackson
Hello Amit, Are you using the public Main Galaxy instance at http://main.g2.bx.psu.edu (usegalaxy.org) or another server specific to your project? If you are working with a project's Galaxy server, then you will need to contact the administrator running that instance. It sounds as if you are

[galaxy-user] The last column in the output file of 'Screen Motif'

2012-10-17 Thread Yunyi Zhu
Hi, I was using the tool 'Screen Motif' to find a particular motif in given region of genome. I don't know what dose the last column in the output file stand for. My guess is that it's -10*log(p), is it? -- 朱云依 Yunyi Zhu 中国科学技术大学University of Science a

Re: [galaxy-user] How to merge Fastq groomer files in Galaxy?

2012-10-17 Thread Peter Cock
On Tue, Oct 16, 2012 at 8:22 PM, Fang,Xiefan wrote: > Dear Galaxy users, > > Does anyone know how to merge several FASTQ groomer files by using > Galaxy? If not, is there any other program that can achieve this? The size > of one FASTQ groomer file is around 1GB. Thank you! > The Galaxy

[galaxy-user] How to merge Fastq groomer files in Galaxy?

2012-10-17 Thread Fang,Xiefan
Dear Galaxy users, Does anyone know how to merge several FASTQ groomer files by using Galaxy? If not, is there any other program that can achieve this? The size of one FASTQ groomer file is around 1GB. Thank you! Best regards, Xiefan Fang, Ph.D. Postdoctoral associate Department of Pe

[galaxy-user] Error installing MACS on local Galaxy

2012-10-17 Thread Jerzy Dyczkowski
Hello, I cannot run MACS on the local Galaxy. When I install MACS version 1.4., it gives error something like "cannot find macs". When I install version 1.3.7.1 it gives error "/line 34, in from MACS.OptValidator import opt_validate ImportError: No module named MACS.OptValidator"/ I sourced

[galaxy-user] SNP Calling

2012-10-17 Thread Francesco Vitiello
Does anyone knows a step-by-step pipeline to SNP calling on illumina dataset? >From the alignment to the end thanks Francesco -- ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the publ

[galaxy-user] FW: problem in data uploading

2012-10-17 Thread Amit Maiti, PhD
Hi, I am a first time user in galaxy for analysis of my RNA-Seq data. I am facing problem in loading data in galaxy. 1.I successfully transfered fastq files (from Illumina) by FTP and then tried to upload by checking each files and execute. 3. Initially it was yellow (with rotating c

[galaxy-user] cuffdiff variance calculations

2012-10-17 Thread i b
Hello forum, I was reading some of the theory behind cuffdiff and how it calculates for DE genes. My question: if I do not have replicates, how does cuffdiff do the test statistic which is dependent on the variance? The manual says "Note that in order to calculate the test statistic T, we need to