[galaxy-user] upload more than 2GB data from local computer to local installation of galaxy

2014-02-24 Thread do kadya
Hi, I am unable to upload more than 3 GB data from local computer to local installation of galaxy. How can I do that ? I saw many of the tutorials which says about online upload. My Galaxy is running on 127.0.0.7:8080 I tried FileZilla to upload file, but its says Connection established, waiting

Re: [galaxy-user] upload more than 2GB data from local computer to local installation of galaxy

2014-02-24 Thread do kadya
How can I create ftp for my local computer to upload data for local galaxy installation ? For current I may can use normal text upload but if I am having 10-20 GB file then I will need FTP upload. Waiting for positive reply. Hi, I am unable to upload more than 3 GB data from local computer to

Re: [galaxy-user] upload more than 2GB data from local computer to local installation of galaxy

2014-02-24 Thread Björn Grüning
Hi Do, If you are running a loacl instance and you are admin you can use the data library and upload or even better link your data into a data library. https://wiki.galaxyproject.org/Admin/DataLibraries/UploadingLibraryFiles Cheers, Bjoern Hi, I am unable to upload more than 3 GB data from

[galaxy-user] Variant annotation in galaxy

2014-02-24 Thread garzetti
Hallo Galaxy users, I would like to annotate variants (in vcf file) found in my bacterial genomes and look which of them cause non-synonymous mutations. I have found two tools in the Main Galaxy that I can use for this purpose (snpEff and Annovar), but I have problems with them. How can I

[galaxy-user] Converting VCF to gd_genotype

2014-02-24 Thread Mike Montague
I'm interested in using the Genomic Diversity tools. I've used these with success for a previous project. For this current project, I've converted a vcf file (29 subjects) to a gd_genotype file. When I attempt to specify individuals, the checkboxes for each subject are not present. Without

Re: [galaxy-user] upload more than 2GB data from local computer to local installation of galaxy

2014-02-24 Thread Martin Čech
Hello, Björn is exactly right. Link the data to Galaxy as described on the wiki page he posted. If you want to run FTP server with Galaxy (it is not included by default) you have to install and configure it yourself. Here is a wikipage that will show you how to do it.

Re: [galaxy-user] Converting VCF to gd_genotype

2014-02-24 Thread Jennifer Jackson
Hello Mike, Yes, we have similar recent reports of issues with tools from this group and are actively investigating the root cause. It is unknown whether or not python version is related, but right now we are tracking the issues under a single ticket with that as a suspected contributing

Re: [galaxy-user] Variant annotation in galaxy

2014-02-24 Thread Jennifer Jackson
Hello Debora, There are no current plans to include additional genomes to the SnpEff tool on the public Main Galaxy instance at http://usegalaxy.org. For the ANNOVAR tool, the supported genome at this time is hg19. When a .vcf file is assigned to that reference genome (aka database), the

[galaxy-user] large data to upload problem

2014-02-24 Thread do kadya
Hi, Thank You for answers, I am able to learn everyday. I am having 500 GB hard disk, I am trying to integrate 'x' tool to galaxy for local lab purpose. 'x' tool require bam files which are of 400 GB file size. When I used Admin section to upload file my computer hang because of no space. Is it

Re: [galaxy-user] large data to upload problem

2014-02-24 Thread Björn Grüning
Hi Do, the link I send you yesterday, contains a section about linking file. Have a close look at the upload form in the admin section. There should be an option to link the file into Galaxy. As a side note, I really think you will need an additional harddrive, 100GB free space would be not