Re: [galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-31 Thread Yupu Liang
Using full path in the ini file fix the problem! Thanks, Yupu On Jan 30, 2014, at 10:21, Jennifer Jackson wrote: > Hi Yupu, > > I'm sorry, I mixed up the processing for custom builds with the processing > for installed builds in my initial reply. A .2bit & .len file are appropriate > for what

Re: [galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-30 Thread Jennifer Jackson
Yupu, Very glad! The thanks goes to Jeremy who sorted out the issue. We had some big changes the last few release cycles, and unexpected issues popped out. It was very nice of you to not only take the time to report the problem, but to also follow up with sending feedback until resolved. If

Re: [galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-30 Thread Jennifer Jackson
Hi Yupu, I'm sorry, I mixed up the processing for custom builds with the processing for installed builds in my initial reply. A .2bit & .len file are appropriate for what you are doing - and the wiki instructions are accurate. Still, I double checked with Jeremy, the scientist that developed

Re: [galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-29 Thread Jennifer Jackson
Hello Yupu, Using a .len file is problematic at this time. In the upcoming release this will be corrected. Using a .fasta file is the solution. In many cases, using a .fasta file can be preferred as it will include the reference genome sequence in the visualization, but the choice is yours, o

Re: [galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-29 Thread Yupu Liang
Yes I have restarted my Galaxy many times and I have also tried different genome build such as hg18 and experience the same problem. Yupu On Jan 29, 2014, at 3:32, Hans-Rudolf Hotz wrote: > Hi Yupu > > > Just double checking: Have you restarted your Galaxy server after adding the > len files

Re: [galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-29 Thread Hans-Rudolf Hotz
Hi Yupu Just double checking: Have you restarted your Galaxy server after adding the len files? Regards, Hans-Rudolf On 01/28/2014 08:12 PM, Yupu Liang wrote: Hi, I am trying to set up the visualization functionality on our local Galaxy installation. I followed the instructions on htt

[galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-28 Thread Yupu Liang
Hi, I am trying to set up the visualization functionality on our local Galaxy installation. I followed the instructions on https://wiki.galaxyproject.org/Visualization%20Setup And here is the list of len files: ls -l tool-data/shared/ucsc/chrom/ total 200 -rw-rw-r-- 1 galaxy galaxy10 Jan 2

Re: [galaxy-user] visualization problems

2013-06-13 Thread Ann Holtz-Morris, M.S.
Hi Jen, I just tried it again. Just in case I cleared my browser, Firefox, cache, history and cookies, although not memorized passwords. The saved visualization gave me the Guru mediation error , GURU MEDITATION: #202b9e1bd2a8460a8089153f0853e7f4, and when trying to visualize a bam file I g

Re: [galaxy-user] visualization problems

2013-06-12 Thread Jennifer Jackson
Hi Ann, I was not able to duplicate the behavior, but the way visualizations are launched and the time frame in our logs are both a fit with an earlier known job scheduling delay on the public Main instance. Please try the visualization again, if you haven't already, and let us know if it is

[galaxy-user] visualization problems

2013-06-12 Thread Ann Holtz-Morris, M.S.
Hi, Earlier today I couldn't add a new visualization. I just was working again and now I cannot load the viualizations that I'd previously set to load (BAM from SAV). I get the message Internal Server Error Galaxy was unable to sucessfully complete your request GURU MEDITATION: #ff39a25

Re: [galaxy-user] visualization

2011-11-09 Thread Jennifer Jackson
Hello Peggy, The reference genome named simply "arabidopsis" in Galaxy is a legacy genome name and may not work with all tools. Assigning the reference genome name "Arabidopsis_thaliana_TAIR9" instead is the recommended choice (both are TAIR9). The next item to change is the format of the ch

[galaxy-user] visualization

2011-11-09 Thread wu yijin
Hi All, I am new of Galaxy. I want to visualize my short mapping results in the browser. I uploaded my .wig file which has format as this, (first column is position, second is the read soverage of that position) variableStepchrom=Chr1 4 1 5 1 6 1 7 1 8 2 9

Re: [galaxy-user] Visualization > New Track Browser dropdown menu too long

2011-09-06 Thread Farhat Habib
You can just type the first few letters of your organism to go through the list quickly. -Farhat -- Farhat Habib, PhD Scientist-C, Center of Excellence in Epigenetics Indian Institute of Science Education & Research, Pune http://www.iiserpune.ac.in/~farhat Twitter: @far_hat Office: +91 20 2590 80

Re: [galaxy-user] Visualization > New Track Browser dropdown menu too long

2011-09-06 Thread Kevin Lam
Oh btw I just looked at the list of loaded system-installed builds. there are 934 genomes if I am not wrong. browsing 5 by 5 is quite excruciating! On 6 September 2011 21:00, Kevin Lam wrote: > I found the dropdown menu list to be too long to be effectively used > especially when viewed i chrome

[galaxy-user] Visualization > New Track Browser dropdown menu too long

2011-09-06 Thread Kevin Lam
I found the dropdown menu list to be too long to be effectively used especially when viewed i chrome (browser) I can only see 5 genomes at a time and the ordering of the genomes isn't intuitive. Can I suggest that a frequently used genomes list be in the rest of the page so that it is easier to use

Re: [galaxy-user] visualization of alignment

2011-08-29 Thread Peng, Tao
===> Please use "Reply All" when responding to this email! <=== Hi Jen, I have been using the following link for learning to visualize TOPHAT results: http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise How can get the RefGENE for the whole human genome instead of Ch19 listed

[galaxy-user] visualization of alignment

2011-08-18 Thread Peng, Tao
Hi jen, I have used BOWTIE to align my RNA-seq reads to HSV2 genome; out of 35,000,000 lines, only 621 lines left when I chose to have mapped reads only. How can visualize these aligned reads to HSV-2 genome? In the panel of converted SAM to BAM, I tried to use the data in trickster, but I am not

[galaxy-user] visualization of alignment

2011-08-18 Thread Jennifer Jackson
Hello Tao, For the Bowtie results, the aligned results may be low because the data is RNA and not DNA. TopHat is generally considered a better choice for RNA since it allows for bridges over splice sites (introns). The full documentation for each program is on each tool's form and/or you can

Re: [galaxy-user] visualization

2011-08-04 Thread Jeremy Goecks
ck on: 'Visualization -> 'New Track Browser' -> 'Add a Custom Build' > > > Hope this helps. > > Regards, Hans > > > On 08/04/2011 01:51 AM, vasu punj wrote: >> IGV should allow you to do this but not sure about trackbrows

Re: [galaxy-user] visualization

2011-08-04 Thread Hans-Rudolf Hotz
allow you to do this but not sure about trackbrowser in Galaxy. Vasu --- On Wed, 8/3/11, Jiannong Xu wrote: From: Jiannong Xu Subject: [galaxy-user] visualization To: "galaxy-user@lists.bx.psu.edu" Date: Wednesday, August 3, 2011, 6:03 PM Hi Jen, I mapped illumine reads to draft gen

Re: [galaxy-user] visualization

2011-08-04 Thread Nicola Nadeau
I use IGV outside of Galaxy. If you download your bam and bam index files you can load those and your reference into IGV to visualise them. On 04/08/2011 00:03, Jiannong Xu wrote: Hi Jen, I mapped illumine reads to draft genomic contigs, and try to visualize the mapping. Is there any way I ca

Re: [galaxy-user] visualization

2011-08-03 Thread vasu punj
IGV should allow you to do this  but not sure about trackbrowser in Galaxy. Vasu --- On Wed, 8/3/11, Jiannong Xu wrote: From: Jiannong Xu Subject: [galaxy-user] visualization To: "galaxy-user@lists.bx.psu.edu" Date: Wednesday, August 3, 2011, 6:03 PM Hi Jen, I mapped illumin

[galaxy-user] visualization

2011-08-03 Thread Jiannong Xu
Hi Jen, I mapped illumine reads to draft genomic contigs, and try to visualize the mapping. Is there any way I can use my own reference contigs for visualization? Thanks John Xu NMSU ___ The Galaxy User list should be used for the discu