[Histonet] NSH Blog

2008-09-11 Thread Della Speranza, Vinnie
I didn't see this announcement posted on the list and thought it might be of interest to some. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 FYI BLOGGING NSH

[Histonet] formalin-formula and osmolarity

2008-09-11 Thread Gudrun Lang
Hi all, I need the help of those with a talent in chemical calculation. This formula is used in our institute since 1970, but nobody knows the origin or a publication. Formalin-formula: 8% buffered Formaldehyd 2 Liter 36% Formaldehyd 2 Liter Phosphatpuffer pH 7,8 6 Liter Aqua dest. EndpH

Re: [Histonet] Protocol: Saving unstained slides...

2008-09-11 Thread Greg Dobbin
Hi Daniel, All of our blanks are saved for a short time (ie 2-4 weeks) just in case, but those from cases that have very little tissue left in the block or small malignant foci are filed with the HE slides (and any other stained slides of course) that go with the case. The idea is that if in 2

[Histonet] Pig endothelium

2008-09-11 Thread Alistair Lindsay
Dear All, Does anyone have any experience with staining pig endothelium, specifically keeping rings of blood vessels alive? I am collecting fresh tissue from the slaughterhouse and incubating it with tumour necrosis factor to induce VCAM-1 expression. However, I suspect that the tissue quickly

RE: [Histonet] Pig endothelium

2008-09-11 Thread Weems, Joyce
Lipstick, maybe? Sorry, couldn't help it... j -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Alistair Lindsay Sent: Thursday, September 11, 2008 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pig endothelium Dear All, Does anyone

Re: [Histonet] Pig endothelium

2008-09-11 Thread Mark Tarango
Are you putting the tissue into media like RPMI or DMEM to keep it viable until you use it? P.S. Joyce, I loved the lipstick comment hehe On Thu, Sep 11, 2008 at 11:42 AM, Alistair Lindsay [EMAIL PROTECTED] wrote: Dear All, Does anyone have any experience with staining pig endothelium,

[Histonet] RE: Histonet Digest, Vol 58, Issue 13 - RECYCLERS

2008-09-11 Thread Kay, Karen
Hello, We have used the CBG product for a few years now and are very happy with both the equipment and support/service. The recycler is simple to use. We have been able to fit it into the tasks of the day. It has been a very reliable instrument. The staff are always willing to help whenever we

Re: [Histonet] bottom of water bath

2008-09-11 Thread Geoff McAuliffe
You can paint stainless steel if you prepare the surface and prime correctly. Call the help line of Benjamin Moore, Sherman-Williams, Zinsser or ?? and tell them what you need. Geoff DiCarlo, Margaret wrote: Hello Histonetters, Working in a Bone Lab, I cut large sections which often

RE: [Histonet] bottom of water bath

2008-09-11 Thread Pamela Marcum
Margaret, Look for a silicone microwave oven mat and cut it to size. They come in colours and should lay nicely on the bottom. If need be it could be weighted down along the sides with fishing weights or even a small metal bar cut to size. It is easier than painting and makes cleaning a breeze