[Histonet] Humidity levels in the lab

2008-09-18 Thread Randolph-Habecker, Julie
Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or pl

[Histonet] Travel/Temp position in Beautiful Orange County, California

2008-09-18 Thread Kyla Nemitz
Hello All - One of the facilities I work closely with has asked me to search for a travel histo tech. A facility in Orange County, CA needs a contract ASCP histo tech for a 13 week travel assignment starting somewhat as soon as possible. I specialize in placing histo techs on travel and p

[Histonet] Cassette Holders

2008-09-18 Thread histoinfo
Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows t

[Histonet] Ventana Retic Stain

2008-09-18 Thread Laurie Colbert
Is anyone out there having problems with their Retic stain on the Ventana Nexus Special Stainer? Please contact me offline. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/h

Re: [Histonet] Helicobacter

2008-09-18 Thread Cynthia Robinson
We keep track of our positive cases and only cut one ribbon for use as a positive control. This way we don't use up the block. Cindi >>> Rene J Buesa <[EMAIL PROTECTED]> 09/18/2008 10:51 AM >>> The reason why the pathologists are usually reluctant to use (+) cases, is because if they are used a

Re: [Histonet] Helicobacter

2008-09-18 Thread Jennifer MacDonald
We used to cut just a number of slides from each positive block that we had. This way no block was exhausted in the process. Rene J Buesa <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 09/18/2008 08:55 AM Please respond to [EMAIL PROTECTED] To histonet@lists.utsouthwestern.edu, Matt Roark

Re: [Histonet] Helicobacter

2008-09-18 Thread Rene J Buesa
The reason why the pathologists are usually reluctant to use (+) cases, is because if they are used as a (+) controls they will be probably used up totally within a short period of time and IF there is some sort of legal action against them or the hospital in one of those cases there will be no

RE: [Histonet] ?bacterial/fungal contamination on slides

2008-09-18 Thread Laurie Colbert
We see this every once in a while, and have traced it to the water stations on our stainer. They are supposed to be cleaned with bleach once a week, but that wasn't happening. Another time, we had contamination on the spigot of our 5 gallon deionized water bottle. We are supposed to clean that w

RE: [Histonet] ?bacterial/fungal contamination on slides

2008-09-18 Thread Tom McNemar
We had a similar problem awhile back and traced it to our precut control slides. We cut certain control slides ahead and store them in slide boxes. We started seeing fungus where it should not have been. We were just cutting the control slides, putting the wet slides into the boxes, covering

Re: [Histonet] Helicobacter

2008-09-18 Thread Matt Roark
Thanks everyone! I also thought that we should be using positive patient controls but our pathologist wasn't to keen on the idea for some reason. But now with all of your responses maybe we will be. Thanks again! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center

[Histonet] Re: Helicobacter stain control

2008-09-18 Thread Robert Richmond
Since gastric biopsy specimens with abundant Helicobacter are rather common, it's easy to find Helicobacter controls among your own specimens, and almost every laboratory I've worked in has obtained controls out of their own material. When I see a case that would make a good control (not every posi

[Histonet] ?bacterial/fungal contamination on slides

2008-09-18 Thread Beverly Rowe
We are experiencing contamination of GMS and PAS-F stained slides with a filamentous microorganism, bacterial or possibly fungal. Our flotation baths and stainers are cleaned daily with Sparkleen and water. We use distilled water to fill the baths. We use the Ventana system for the stains, which i

[Histonet] RE: Histonet Digest, Vol 58, Issue 20

2008-09-18 Thread Versfeld, BG, Mr <[EMAIL PROTECTED]>
can someone please provide me with a recipe for hirschprung's disease. thanx. From: [EMAIL PROTECTED] on behalf of [EMAIL PROTECTED] Sent: Wed 2008/09/17 07:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 20 Send Histonet mai

[Histonet] mouse monocytes isolation

2008-09-18 Thread Carol Milligan
I would like to make some electrophysiological recordings from mouse monocytes using an automated planar patchclamp system. The problem is, that I need at least 1x106cells /ml to do this. I have never isolated monocytes from mice so I would like to locate a lab in the UK who routinely isolate pure