[Histonet] Openings Minneapolis Area

Is anyone in need of or aware of Histotech positions in the Minneapolis area. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block

Peroxidase is an enzyme. It doesn't miraculously appear as a result to exposure to heat. You show me one one slide where this has occurred and I will humbly remain silent in this forum for a whole month! Ok, maybe just a week... :-) Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology D

[Histonet] paraffin sectioning problems

I recently started paraffin sectioning and I am having trouble with getting good quality sections reproducibly. I was wondering if you have any tips that may help. I am sectioning thymus, pancreas, and lung. Section thickness is 10 um. The microtome that I am using is Reichert-Jung Supercut, dis

[Histonet] Re: Which one first - antigen retrieval or peroxidase block

I'm with Rene on this one. Block after retrieval. For those of you who do the block before retrieval, how do you control for any activity that might be exposed during the retrieval step? Couldn't it lead to false positives in your slides? Albert C. Grobe, PhD Tissue Engineering Lab In

RE: [Histonet] JOB OPENING

Sorry, I should have said three shifts starting at 6:00am, 7:00am, and 7:30am. With a late Friday 9:30am - 6:00pm once a month. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Joyce Cline Sent: Monday, September 29, 2008 3:37 PM To: Histonet Subject: [Hist

[Histonet] cryostat

Hello, We are looking for a good refurbished cryostat for a back up. Our back up has gone caput. Anyone know of any. E-Bay has some. Robyn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listin

[Histonet] SV40 antibody

I need some help with getting sv40 to work in paraffin. Will someone share there source and protocol. Thanks in advance. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ___ Histonet mail

[Histonet] sv40 antibody

I need some help getting sv40 to work in paraffin sections. Will someone share a source and protocol. Thanks in advance. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ___ Histonet mail

[Histonet] JOB OPENING

I have an opening for a full time Histotechnican. M-F 6:00am to 4:30pm. We are a rural hospital located in Hagerstown, Md. The lab is part of the for-profit side of the organization. We do about 17,000 cases a year. We are one hour from Baltimore and Washington D.C. Call our H.R. department at

Re: [Histonet] 4% Paraformaldehide

Hi Ben: Do you mean rate of penetration, speed of fixation or the actual concentration of formaldehyde in the solution? Remember, formaldehyde penetrates tissue rapidly BUT fixes tissue slowly. Geoff Ben Spirto wrote: How can I measure the strength of 4 % paraformaldehide? It seems that our

Re: [Histonet] 4% Paraformaldehide

Hello,   What species and how long are you fixing?   Paula - Original Message From: Ben Spirto <[EMAIL PROTECTED]> To: Histonet@lists.utsouthwestern.edu Sent: Monday, September 29, 2008 1:01:01 PM Subject: [Histonet] 4% Paraformaldehide How can I measure the strength of 4 % paraformald

RE: [Histonet] Freezing tissues

Saro, Just a thought, could you use a clear water soluble microbiology gelatinous plate? Never done this, but it would keep them standing on edge. Robyn -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Bascaramurty, Saro Sent: Monday, September 29, 200

[Histonet] 4% Paraformaldehide

How can I measure the strength of 4 % paraformaldehide? It seems that our lab made 4% para does not adequately penetrate the brain tissue. Any suggestions? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] Rapid tissue processors

We are in the process of looking for a rapid tissue processor. The one's we are looking at are: Leica Peloris Rapid Tissue Processor Tissue-Tek Xpress x50 (Sakura) STP 420D Tissue Processor (Microm from Richard-Allan Scientific)/Thermo Fisher Scientific RHS-1/RHS-2 Rapid Microwave Histopro

RE: [Histonet] Plus Slides

Me too! Me too! I also buy my plus slides from Mercedes. They sell a few different types. I buy the Starfrost Platinum line. Not only are they affordable, they are very high quality slides. I don't usually even have a problem with dye uptake. Call them and ask for a sample. Have a great week. Jenn

[Histonet] Freezing tissues

Hello, Does anyone out there have a good method to freeze animal vessels in order to have good morphology/free of artefacts etc. for some spectroscopic studies and histochemistry? Using liquid nitrogen is ok, but not isopentane or any other chemicals that would interfere with the spectra. Thank

[Histonet] amoebae

Has anyone ever used PAS Mentanil yellow for staining amoebae. Sharon Genest MLT Technologist Supervisor Histology Saskatoon Health Region Phone: (306)655-8197 Email: [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://

RE: [Histonet] Plus Slides

I also use slides from Mercedes, don't see dirt debris but I do see that the slides take up the hematoxylin stain, it does not seem to cause a problem though. Mercedes sells the cheapest charged slides I can find anywhere. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 1

[Histonet] Block "shippers"

Recently we have had paraffin blocks shipped to our facility in clear, four chambered carriers. Can anyone tell me where I can get these? Thanks, Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 [EMAIL PROTECTED] (801)

Re: [Histonet] blue paraffin

At arts and craft stores (Michael's, Ragshop) that sell supply for candle making, they also have special coloring agent for paraffin/wax. - Original Message - From: "Robyn Vazquez" <[EMAIL PROTECTED]> To: "FU,DONGTAO" <[EMAIL PROTECTED]>; Sent: Monday, September 29, 2008 10:48 AM Subj

RE: [Histonet] blue paraffin

Hello, Maybe try some blue food coloring and stir it up. Never tried it though. Robyn -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of FU,DONGTAO Sent: Monday, September 29, 2008 7:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blue paraff

RE: [Histonet] Plus Slides

We buy our slides from Mercdes, usually do not have a problem with them Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Meghan Tucker [EMAIL

RE: [Histonet] blue paraffin

We get ours from Surgipath. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 [EMAIL PROTECTED] The information contained in the message and the documents accompanying this message contain information that is privileged and co

[Histonet] blue paraffin

Hi, all Does anyone know any vendors sell a blue paraffin? I've heard it will help to find some small tissues? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] blue paraffin

Surgipath does. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of FU,DONGTAO Sent: Monday, September 29, 2008 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blue paraffin Hi, all Does anyone know any vendors sell a blue paraffin? I'

RE: [Histonet] Plus Slides

Hi, I would contact the vendor as they are usually resellers of the slides not the makers and need to know it there is a problem. We use Thermo Colorfrost and have good luck. Occasionally we do have a few bad slides as you will find with all vendors. I always call them and we get it fixes with

[Histonet] Plus Slides

Good Morning!   I was wondering where labs purchase their Plus slides from and also if anyone is having trouble with debris on their slides?  We have been having a lot of trouble with dirty slides right out of the box.   Thanks!   Meghan Tucker   ___

[Histonet] Positions Available

The University of Texas M. D. Anderson Cancer Center in Houston, Texas has two Histology Technician positions available in the Frozen Section Laboratory. To apply go to www.mdanderson.org or call Dianna Menard @ 713-745-6184. Kaye Barr HT (ASCP) Laboratory Manager