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Hi,
I need to detect particles that are covalently labelled either with
fluorescein or carboxytetramethyrhodamine in rat liver using paraffin
sections. Could someone please suggest the best way of doing this.
Thank you,
Rema
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Rema Oliver PhD
Research Fellow
Surgical & Orthopaedic Resear
Because I work in small lab, we currently buy everything pre made. Prior to
that all I used to do was write my received day on by dry chemicals, open date
and either write stable or no expiration date on the label. To date I have not
had an issue with it for either CAP or CLIA.
Tom Podawiltz,
Anita:
Dry chemicals, especially INORGANIC are natural products that exist in nature
for millions of years and we just extract them. It is unwarranted to have an
expiration date on them.
Another thing is the condition of being anhydrous, and the periodic acid that
you mention is a good example o
Do I have any takers for my question about staining for sulfur
deposition in the brain of a sheep? Anybody? Help?
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
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We have been working with ours for about a month now and its nice.
Takes a bit more with respects to programming the runs, but we are using
it as an open system since we are in research and work primarly with
animal tissue. But overall we really like it.
LIz
Elizabeth A. Chlipala, BS, HTL(ASCP
I am looking for some feedback on the Dako autostainer link (newest machine)
and/or the Dako ACIS III slide imager. Our lab is negotiating a contract and I
need some ammo to fight for or against it. Whatever the case may be.
Thanks in advance.
Loralee McMahon, HTL (ASCP)
ICC Supervisor
Unive
I have powdered stains that are probably 10 years old. I date dry chemicals
when received and put a 10 year expiration date, if no expiration date is
provided by the company. This has satisfied all my inspectors so far. I have
discarded all the stains and chemicals we do not use anymore through a l
We created an access database file (you could also do it on an excel
spreadsheet) with all the dry chemicals. We included date received, date
opened, chemical catalog #, name and expiration date. We assigned expiration
dates based on our best knowledge of how long it would be good. We then so
Dear Histonetters,
I'm looking for methods to preserve intestinal tissue samples, in order
to later isolate RNA from laser microdissected cryosections.
I'm a bit worried about the intestinal enzymes (not sure if the RNA will
be preserved even at -80C for very long). I was told that RNALater mig
hello all, we were inspected the beginning of june by cap and one of our
deficiency was anp.21382. it had to do with outdated reagents. my director
want all of my dry chemicals gone I have gone through them and just have
what I need for the special stains that we do in house. does anyo
Hi Dave,
I noticed that you posted a request in march of 2004 on histonet for a
Sirius red fast green staining protocol. If you do have a protocol, would
you mind sending it to me. I have had a hard time finding one to compare to
mine and my lack luster results.
Thank you,
Adam Bazam
One of our students is interested in staining sheep brains for sulfur -
is there a definitive stain for this? I've done some basic searching but
come up empty. Thanks.
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-
Good morning out there in histoland,
I am looking for some help in finding two antibodies:
- sulfated gastrin-17 (G-7)
- sulfated cholecystokinin-8 (CCK-8)
All the antibodies that I are finding are non-sulfated.
Thank you all for your help,
Eva Permaul
Georgetown University
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Dorothy:
At the end you are going to use tissues from your laboratory to do the tests,
and you do not know how other labs processed their tissues. Although it is not
absolutely clear how the processing steps (other than fixation) can affect the
reactivity of a tissue, it is always better not to
Is it better to use a lab's own tissue for validation in a microarray
for Ihc purposes or would it be passable to use TMA's from another
source?? Thanks for your opinions!!
Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Pa
Hi, adjust the gelatin concentration!
Our experiences of brain section (95 degree boiling for 30 min): 0.5%
gelatin-coated slides coming off sections, not for 0.25%.
Also, try to use "floating sections" rather direct attach frozen sections
embedded with O.C.T on to slides.
2008-10-06
tf
We don't use it for processing but we use it at the end of the stain set
up and coverslip out of it using Clearium mounting medium (it is misable
with alcohol). We did it to keep out fingers out of xylene as it is a
neurotoxin.
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PRO
I work with non-decalcified and decalcified bone. After I have tried to
pressure cook it, steam it, microwave it, etc my sections look pretty
bad(coming off, etc.) I have found that either using Pepsin (Zymed) for 20
minutes at 37 degrees C. in a humidifying chamber or Pepsin for 20 minutes at
room
A full time HT position is coming available at Mission Hospitals in beautiful
Asheville, NC.
Contact me for more information!
Jeanne
Jeanne Clark HT/MLT (ASCP)
Pathology Manager
Mission Hospitals
428 Biltmore Ave
Asheville, NC 28801
828-213-5169 office
423-612-1213 cell
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