Thanks to those who answered, those who were serious and those who weren't
:-) Now i can sleep easier, nights. Anyway its all the fault of
spellcheckers (which is satining got in..to those who picked it up!)
love u all
On 10/15/08, Jacqui Detmar [EMAIL PROTECTED] wrote:
Hey there. I'm in
Thanks to everyone who replied to my query! I received many insightful
responses.
Merced
--On Thursday, October 09, 2008 5:19 PM -0400 Merced Leiker
[EMAIL PROTECTED] wrote:
What are people's exerience with manual processing of FFPE tissues using
a microwave? My boss has me looking into
I am looking for a protocol to fix and stain in paraffin embedded
subcutaneously grown mouse tumors for LacZ. Can anyone help? M.
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Melissa wrote:
I am looking for a protocol to fix and stain in paraffin embedded
subcutaneously grown mouse tumors for LacZ. Can anyone help? M.
Far as I know, one cannot stain paraffin embedded material with x-gal. We will
do either whole mount X-gal staining, post-fix, and then paraffin
For full time permanent positions
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With employment opportunities all over the US, we
Hazel, we are an information system vendor who has built their entire
computer system around LEAN. If you'd care to hear any details please email
me outside the group.
In a nutshell, you never have to enter a case # in the system.
Michael Mihalik
PathView Systems | cell: 214.733.7688 |
Dear Markus,
We do X-Gal staining on bone and in the best scenario the animals are
perfused with glutaraldehyde (GA). PFA tends to quench the beta-gal
activity and the results may not be 100% accurate (underestimation of
positivity). Though if you want to do IHC on these samples, I am
sure
Our PSLIM died before it ever made it to production testing. Our
developer finally got some nice looking slides to print with a 2D bar
code. Then for some unknown reason it jammed and had to be returned.
They are sending a new unit with updated software.
It has potential but can't be jamming
Dear Histonetters,
You may recall my earlier post on rings of pig aorta. I have managed to
keep them alive quite nicely with DMEM many thanks for the tips.
Unfortunately, I am still struggling to stain for endothelial adhesion
molecules.
I have read and received various different opinions on
Hello everyone,
Does anyone have a good Von Kossa stain protocol that they would not mind
sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA or
MMA (sections are between 5 and 10 µm thick)? I would appreciate it greatly.
Thank you much.
Jim
Speaking of expiration dates. What does everyone do with their powdered
reagents like hematoxylin, ferric choride etc. etc. that dont have an
expiration date on the bottle. How do we determine the shelf life? What is the
rule? I was always taught as long as it worked it was okay. Is that a
Jim,
Here you go:
MMA Thin (5 microns) Sections
1) Deplastify with three changes of Xylenes @ 60C for 30-60 minutes (depending
on size of section). You can also use three changes of acetone @ RT for 15-30
minutes.
2) Hydrate tissue from ethanol to DI water (100% EtOH for 5 min, 95% EtOH
Hello,
I'm looking for a myoglobin antibody that reacts in rat tissue and works
in FFPE tissue. Can anyone recommend a vendor?
Thanks for any info.
~Michele
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