Does anyone use agar in a procedure to prepare cell blocks from cytology
material? I am interested in such a procedure as a method to help
eliminate/reduce floaters in cell block preparations?
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL
I have a question about a Leica Autostainer XL. We are getting the most
peculiar odor from it during staining and none of us can quite describe it.
One of our histotechs is very sensitive to it however. We have changed the
filters and the odor is still there. Also, the arm mechanism is very
In my experience, you can use the Biocare Decloaker with their default program
for most protocols (I believe it heats @ 125C for 10 seconds and then 90C for
10 minutes, but dont quote me on that because I am not sure). I do not preheat
my buffer when I use the pressure cooker, since it heats
With the new CAP regulations concerning Her-2/neu and Predictive Markers on
breast specimens, has anyone let the processed specimens sit at room temp
for a length of time before embedding? We are not a 24/7 staffed lab and
would appreciate any input on how others deal with these regulations.
I'm wondering if anyone out there has tried something called Uni-Trieve from
Innovex Biosciences that claims to be a universal low temperature retrieval
solution ie: 75 degrees for 15 minutes. I wouldn't mind trying it on bone
sections to see if they stay on better.
Larry A. Woody
Seattle,
We have also noticed that we get preciptate and mold in the water drain
hose. We bleach it once a month along with the water containers. Our hose is
not smooth (corrugated?)
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Hi Margaret Perry ,
You can put your slides in to cold solution and put in to pressure cooker then
you can set the temperature to 95 C for 12 - 15 min is fine or after reaching
to 95 C you can keep it for 6 - 8 min is good. This protocol is good for when
you use 10% Neutral buffered formaline
