Is the 118 psi a typo? I don't believe the instrument can go that
high-max psi is 30.
Piero Nelva wrote:
I used it for 4 minutes at about 118 psi and most antibodies worked
really well. I was having huge problems with the Er and Her2 and this
solved them (almost) overnight.
-
Isn't the election over?
Griping will do you no good, spambot!
So, being in grant funded research, I hope our new president will put some
money back into NIH.
(I was hoping that no matter who won, so my political views will not spam
you)
Anyone else feel that way?
Emily
--
You would know her
Hi Emily et al.
The President, any President, can put $ for NIH is the budget request
he/she submits to Congress but ONLY Congress can appropriate money. That
is in the Constitution.
Several of my colleagues are out of work due to loss of grant support
'cause NIH, while not broke, has many
Sorry RC, Now who's being biased and where is the fairness? You imply
older histotechs do not study hard and receive a salary that they may or
may not be entitled to?? I go to college and take night classes to keep
from being sterotyped this way. I also have staff that I would stand up
for hands
Greetings,
Can anyone offer a processing schedual for prostate bx cores for the
Leica processor??
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Interesting, the histonet list seems to be occupied by ultraconservatives.
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This kind of material really should not be posted on the HistoNet! Why don't
you find some other place to vent!
Elfi Hacker
Hacker Industries Inc
http://www.youtube.com/watch?v=wFYk7a3vEyo
I don't know how many times I've heard the words Obama and Messiah used
in the same sentence. To
Ditto!
Best Regards,
Mickie
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
Mohs Lab Staffing
Mohs Suite Mohs Reporting Software
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX 509-624-3926
Web: www.mohshistogyconsulting.com www.mohslabstaffing.com
Hi - everyone - does anyone know of any fungi that can not be
demonstrated by PAS stain? We appear to have one in a cow placenta which
is obvious on Grocott and HE but do not stain on PAS. The micro lab
isolated Absidia spp but the hyphae in the Grocott are septate but I
believe Absidia are
Try a CAS stain. = PAS with Chromic acid
Perhaps this fungus needs something stronger than Periodic acid.
Gudrun
-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Weaver,
Colin
Gesendet: Mittwoch, 05. November 2008 18:03
An:
Maybe Aspergillus or Rhizopus.
Larry A. Woody
Seattle, Wa.
From: Weaver, Colin [EMAIL PROTECTED]
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 5, 2008 9:02:37 AM
Subject: [Histonet] Fungal staining
Hi - everyone - does anyone know of any
All I will say on the matter is that if more funding is approved I hope
it is simply pulled from somewhere else and is not in addition
to.because that would mean more taxes!
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
[EMAIL PROTECTED]
-Original Message-
I will be out of the office starting 11/05/2008 and will not return until
11/10/2008.
This kind of material really should not be posted on the HistoNet! Why
are you posting something that's off-topic like this? This is a
Histology list, not a 'I will be out of the office' list! They might
Please receive my apology. It was a provocation
-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED] im Auftrag von [EMAIL PROTECTED]
Gesendet: Mi 05.11.2008 19:09
An: histonet@lists.utsouthwestern.edu
Betreff: Histonet Digest, Vol 60, Issue 9
Send Histonet mailing list submissions to
Hello,
I¹m trying to buy some alcohol for both histology and molecular biology
protocols. But I¹m having some difficult getting ³molecular biology grade²
100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say
anything about the quality. Since it¹s 200 proof, is that basically
Histonetters,
The Texas Society for Histotechnology will be holding it 2009 meeting in
beautiful Austin Texas on May 28-31, 2009 (This is not Memorial weekend) at the
Hyatt Regency Austin Lady Bird Lake, 208 Barton Springs, Austin, Texas.?
The program committee is actively seeking
-Original Message-
From: [EMAIL PROTECTED]
To: histonet@lists.utsouthwestern.edu
Sent: Sun, 2 Nov 2008 7:04 pm
Subject: Texas Society for Histotechnology 2009 Call for Abstracts
Histonetters,
The Texas Society for Histotechnology will be holding it 2009 meeting in
beautiful Austin
Read your secure message by opening the attachment, securedoc.html.
You will be prompted to open (view) the file or save (download) it to
your computer. For best results, save the file first, then open it in a
Web browser.
If you have concerns about the validity of this message, contact the
because that would mean more taxes!
Making more than $250,000/year doing histology? Where do I sign up?! ;)
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We just started doing NONGYN cytology. We have no cytologist. Does
anyone have a suggestion and/or a starting place for how to develop a QA
Plan.
Thanks in advance.
Nancy
~~
This email and any files transmitted
unfortunately, all government funding comes from us peons...that's
the way it has always been and will always be.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
[EMAIL PROTECTED]
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf
Caroline
I would not recommend using the same alcohol for histo that you use for
molecular work. Assuming by molecular you mean isolating DNA/RNA,
cloning, etc. You'll want a higher grade, more expensive alcohol for
nucleic acid work then the histological stuff that comes in a 55 gal drum.
I would like to know how other labs are processing fatty tissue,
especially breast for optimal processing? We have been doing a few
reprocessing of these specimens and my pathologist feels this should
not be happening! Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick,
Dorothy:
Reprocessing fatty tissue (usually a hopeless proposition) is essentially
caused by poor infiltration more than by poor fixation, and poor infiltration
is usually caused by less than optimal time in the clearing agent or antemedium
(usually xylene).
The thing is that fatty tissue
Ooops. One too many ones!
Piero
- Original Message -
From: Linda M Watson [EMAIL PROTECTED]
To: Piero Nelva [EMAIL PROTECTED]
Cc: histonet histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 05, 2008 11:07 PM
Subject: Re: [Histonet] re: Biocare Decloaker HIER
Is the 118
Can you block this person?
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sam Histology
Sent: Wednesday, November 05, 2008 1:09 PM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: bone marrow biopsies
At home, there will be an increase
Even better we will find where he is.
Larry A. Woody
Seattle, Wa.
From: Robyn Vazquez [EMAIL PROTECTED]
To: Sam Histology [EMAIL PROTECTED]; Histonet@lists.utsouthwestern.edu
Sent: Wednesday, November 5, 2008 1:24:58 PM
Subject: RE: [Histonet] Re: bone marrow
Only if he is well fixed first!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW
Altmann's acid fuchsin picric acid is a histological stain for mitochondria.
It requres the tissue to be fixed in a non-acid dichromate fixative, then
post-chromed for some time. The tissue is then processed by paraffin and
sectioned.
Staining is in aniline water saturated with acid
Colin Weaver asks about staining of various fungal species by the
standard fungal stains.
The standard fungal stains have two basic steps:
1. Periodic acid or chromic acid splits bonds between vicinal diol
groups (two carbons with OHs on adjacent carbon atoms) and oxidizes
the free ends to
Hi All,
I totally agree with Robert on his information regarding periodic acid and
chromic acid. I have done extensive research on this subject when I developed
my special stain kits for Biocare Medical. I did incorporate 10% chromic acid
for the GMS stain, and 1% periodic acid for the PAS
Hi,
I looked for you on Reunion.com, but you weren't there. Please
connect with me so we can keep in touch.
-Maria
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