[Histonet] Osteoid identifiction

Hi al, I have received a sample of ?bone where the clinician wants to identfy osteoid. According to my knowledge (now perhaps a little daed) the traditional processing method is plastic embedding of some sort. Is this still the case or are there methods to use standars paraffin processing? Best re

[Histonet] RE: Dako and Leica immunostainers

Sally, I think you answered your own question! You wrote: "If so, why would someone want one of these stainers?" And yet, they sell so many! I have found the Bond to be an excellent instrument and in my opinion it is unrivalled in the market place. I have just recently spent 10 months doing exten

[Histonet] help with Zeiss Apotome

I have inherited a new Zeiss Axiovert system with an Apotome. Our sales rep has helped me to learn the system but he says that Zeiss has no user manual for this microscope and attachments. Does anyone have a protocol for using these instruments, possibly a core facility with multiple users. Than

[Histonet] Experienced temp for third shift needed...

Hi all-- Seeking an experienced temp who likes third shift...this is for a client I REALLY like working with alongside several of our awsome traveling histotemps already in place. (We have GREAT temps!! THANKS GUYS!!) I'll be out of office for a couple of hours tomorrow (eeek! The Twilight m

[Histonet] Dozens of job openings...histotech to histotech

Hi Everyone-- Dozens of openings--all sorts of situations: bench, research, IHC, grossing, supervisor, magagerial...and a few other variations. We have clients in 49 states (if Hawaii comes up I get first dibs!!)--all the jobs you've seen posted here are with labs that work with us, too. So then

[Histonet] tissue/formalin disposal

Hi everyone! Can I get a survey of how higher volume labs (40-50,000 cases per year) dispose of their formalin and tissues? Are there companies out there that will take both (i.e. the tissues remain in formalin in their containers)? My safety officers are asking that we strain the tissues fr

[Histonet] RE: Dako and Leica immunostainers

All, After reading this thread I just had offer my comments. I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps. I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on t

[Histonet] Histology Supervisor- Ohio

I am a recruiter specializing in the laboratory industry. I have a great opportunity in Cincinnati, Ohio, for an experienced Histology Supervisor. Our client is offering competitive salary, great benefits package, and great career advancement opportunities. They are willing to help with relocation

RE: AW: [Histonet] Control slide storage

There has been much discussion on this issue in the past so be sure and check the Histonet Archives. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: [EMAIL PROTECTED] [m

Re: [Histonet] Toe/finger nail help

Matthew: Under separate cover I am forwarding a material I wrote on the subject that I am sure will help you. René J. --- On Thu, 11/20/08, Matthew Lunetta <[EMAIL PROTECTED]> wrote: From: Matthew Lunetta <[EMAIL PROTECTED]> Subject: [Histonet] Toe/finger nail help To: histonet@lists.utsouthwest

[Histonet] immuno set-up

Hello all, I work at a private lab with one pathologist, and one histotech (me). We do about 5,000 cases a year. We are intrested in doing our immuno's in house (right now we send them out), and wanted to know your opinion on the type of equipment that would be best for this situation. Right no

[Histonet] FW: help

> -Original Message- > From: Barone, Carol > Sent: Thursday, November 20, 2008 3:17 PM > To: '[EMAIL PROTECTED]' > Subject: help > Importance: High > > Histo friends...I am working on a five year strategic plan for a Research > Histotechnology Core Lab with very div

[Histonet] Human tissue for research purposes

Hello Histonetters, I have an investigator that is in need of human tissue samples. We would need IBD/UC colon and RA joint/synovium as of right now. Does anyone know where I could locate these tissues? I found three suppliers online, but I was wondering if there are more out there. Do hos

Re: [Histonet] Leica slide writer - problems??

Hi all. I have used a lot of different slides on it too. The best ones I have found are the Starfrost clipped corner slides by Mercedes. The ink looks great and they don't get "stuck" Great price too! Kelly --- On Thu, 11/20/08, Judith L. Williams <[EMAIL PROTECTED]> wrote: From: Judith L. Wil

[Histonet] Slide Printers

Hello Everyone, I am looking for a small slide printer that works off a bar code printed on the cassette. There are some small ones on the market that can sit right next to the microtome for each tech to use. Anyone have any experience with these printers? Thanks! Diane Diane Cadorette-Hall H

Message-ID: <[EMAIL PROTECTED]> [Histonet] ThermoWave users

Roxanne, Here is a protocol that our customers use and like. (None of the microwave times listed include ramp time) 1. Fix your samples 2. Room temperature fix in Preserve or other Formalin free fixative, for 30 minutes (This step is intended if you should decide to use a Formalin free fixati

[Histonet] Positions Phoenix Az

I have 3 full-time positions with 1 possible part-time position available in a state of the art new in-house GI laboratory in Phoenix Az. This is for a 39 man GI group with reaching a goal of 48,000 bx a year with 16,000 IHC a year. Candidates must be ASCP certified, detailed oriented, well orga

AW: AW: [Histonet] Control slide storage

I think the culprit is a "kind of oxidation" the epitopes suffer from. But it would be interesting, if the fixation is a factor of influence, or if the composition of the epitope itself makes the difference. Gudrun -Ursprüngliche Nachricht- Von: Edwards, R.E. [mailto:[EMAIL PROTECTED] Ges

RE: [Histonet] RE: Leica Slide Writer

All of this conversation about ink and slides reminds of the same issue with paper labels. It's not JUST the ink, or JUST the printer, or JUST the label (if using labels). It's ALWAYS about the combination of all the components working together. Michael Mihalik PathView Systems | cell: 214.733

AW: [Histonet] Kappa & Lambda In situ Probes

The producer is Zytovision and the product is called Zytofast. The distributor is Axxora in USA. http://www.zytovision.com/index.html Zytofast Kappa, Lambda or double-stain is a CISH-Kit. They also sell biotinylated or digoxinylated probes. Until now I've no experiences with these products. Gudrun

AW: [Histonet] Stain for Cellulose

This link shows a german website about biological(botanical) stains. There are some, that refer to cellulose-staining. http://www.aeisner.de/index.html Gudrun Lang -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von [EMAIL PROTECTED] Gesendet: Donner

[Histonet] pap pen with vecter antifade

Hi All, I am wondering how people do remove pap pen when they need to cover slip with an aqueous mounting media. Thanks Sam The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privil

[Histonet] Toe/finger nail help

We are having a problem with having fixed, embedded toe/finger nails fall off of charged slides. Are there any tricks out in histoworld in avoiding this problem? Usually it is during the GMS (microwave) staining. the H&E's stay on. We figure it is the heat from the staining process. Any help? t

Re: [Histonet] RE: Leica Slide Writer

We label tens of thousands of slides a year - no paper labels required. I visited a research lab in Germany - they labele 1,000 slides a day. Never had a problem with the ink coming off or disappearing - where would it go? "Patricia Karlisch" <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED]

RE: [Histonet] Leica slide writer - problems??

We have had the same problems, we discovered the slide surface on some of the snowcoat slides changed to a slick surface instead of a rough surface. We have switched to a pale gray slide that still has the rough surface and we have not had any printing wipe off. Joyce Cline, H.T. (ASCP) Technical

Re: [Histonet] Stain for Cellulose

Tom, The alkaline Congo red method for amyloid will also stain cellulose. Examined by polarisation microscopy, only amyloid and cellulose will give the characteristic green dichroic birefringence. In addition, Congo red is fluorescent. Bryan - Original Message - From: <[EMAIL PROTECT

[Histonet] RE: Leica Slide Writer

Just out of curiosity. When the slide writer is working okay, do you still have to place paper labels on the slides or does the ink disappear with time??? Thank you, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code

Re: [Histonet] Stain for Cellulose

Tom, This is a total shot in the dark, but cellulose, being a very large long-chain, complicated polysaccharide, might stain with Grocotts Methenamine silver? You may want to try some different chromic acid oxidation times to try and enhance the cellulose, but get rid of tissue components

Subject: [Histonet] Control slide storage

I have, over the years, seen varying results on this one. Currently, we store everything at room temp except HercepTest (Her-2) slides and ER/PR slides which we now store in the fridge at 2-8 C. I had noticed that when we cut 30 or so in advance, by the time we were getting to the 15th slide o

RE: [Histonet] Kappa & Lambda In situ Probes

Oops... Sorry. Thanks for clearing that up!!! Got my vendors confused! j -Original Message- From: Morken, Tim [mailto:[EMAIL PROTECTED] Sent: Thursday, November 20, 2008 12:38 PM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kappa & Lambda In situ Probes Ju

[Histonet] Immediate Staff Need in Las Vegas

The #1 Recruiter for Laboratory/Biotech Specialists Healthcare Scouts is a medical laboratory/biotech recruitment firm that places professionals in full-time, permanent positions throughout the United States. As the leading nationally recognized permanent placement solution for medical laboratory/

RE: [Histonet] Kappa & Lambda In situ Probes

Just a note, Leica is not affiliated with Lab Vision, but is affiliated with Vision Biosystems. Lab Vision/Thermo Fisher Scientific does not carry lambda or kappa ISH probes. Tim Morken Technical Support Manager Immunohistochemistry Anatomical Pathology Thermo Fisher Scientific -Original

Re: [Histonet] Leica slide writer - problems??

Hi all- we have a Leica and I have tried different slides. here is my take on what works and doesn't: Starfrost Plus or non-plus (German) don't work too well- the pink frosted slides especially - the ink wipes off- the green is better (why?), the white is ok. Unifrost, non charged, by Ever Sc

RE: [Histonet] Kappa & Lambda In situ Probes

Check with Leica (Lab Vision). They knew about the federal changes are were prepared to handle the change. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -O

[Histonet] RE: Histonet Digest, Vol 60, Issue 33 RE LEICA SLIDE WRITER

Hi Sara, The problem may not be with Leica's Slide Writer but with your Surgipath slides. We too have been experiencing issues with our slides which are Surgipath's. Our printer is the Sakura Slide Writer. We have been told here that Surgipath has changed the formalation/manufacturing of the w

[Histonet] Kappa & Lambda In situ Probes

We were using the Kappa and Lambda ISH probes from Dako along with their PNA kit which made them visible under light microscope. Now they have discontinued the Kappa and Lambda probe. Does anyone out there have an alternative that is visible under light microscopy. Thanks Loralee McMahon,

[Histonet] Re: Sheep liver

If you have a veterinary diagnostic laboratory close by or if they are looking in, they should have some sheep liver, hopefully not autolyzed. I am not sure if local slaughterhouses do sheep, but you can call them to find out. Good Luck Gayle M. Callis HTL(ASCP)HT,MT - Original Message -

[Histonet] Stain for Cellulose

I am looking for a stain that will specifically label cellulose, mixed in with other mammalian tisssue elements. Any ideas? Tom Crowell Novartis Institute for BiomedicalReseach Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: AW: [Histonet] Control slide storage

Is itquite as simple as that, I imagine that many epitopes might lose "stainability" if fixation is inadequate or processing poorly controlled. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jackie M O'Connor Sent: 20 November 2008 15:47 To

Re: AW: [Histonet] Control slide storage

CD31 for sure. I do recall most surface markers I used tended to be unstable. Jackie O' "Gudrun Lang" <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 11/20/2008 09:17 AM Please respond to [EMAIL PROTECTED] To cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested

AW: [Histonet] Control slide storage

Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria

Re: [Histonet] Leica slide writer - problems??

Sara Can you give me a call here at Leica and we can discuss. Best regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax [EMAIL PROTECTED] www.leica-microsystems.com

RE: [Histonet] Dako

It really does deparaffinize and retrieve. We have been using it since June. That was a big seller for us and did I say we love it? -Original Message- From: Rathborne, Toni [mailto:[EMAIL PROTECTED] Sent: Wednesday, November 19, 2008 5:31 PM To: [EMAIL PROTECTED]; Josie Britton; [EMA

RE: [Histonet] Control slide storage

I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060

RE: [Histonet] RE: Histonet Digest, Vol 60, Issue 32

Hi Rudy, I may have some. I will look around. Does it have to be fresh, unfrozen? Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Avenue Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTE

[Histonet] Leica slide writer - problems??

Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. T

Re: [Histonet] Processing problems

it might be your processor. Depending on the type of processor, some processors have a rotary valve that rotates from reagent bottle to reagent bottle. When the rotary valves fails, it starts placing a reagent in the reagents bottles indiscriminately. For instance, our processor started spitti