[Histonet] colon cancer

Does anyone know of a type of cancer, either colon or small intestine that is considered hereditary? Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] RE: Dako and Leica immunostainers

Ditto what Amos related and I will add that the companies making these machines are trying to make a profit first and foremost and not necessarily trying to make things easier for us but I am a huge fan of automation when it gets me from A to B more efficiently, with great results and at a reaso

[Histonet] Sakura tape problems

Thanks to all of you who have had problems with the Sakura tape. I have received multiple methods to try to save the sections. When I have tried several and combined some I will let you all know what happened so maybe I can help you. Thanks again, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief

RE: [Histonet] Advanced Histo training

Victor Yes, it was kind of hard to tell exactly what he is seeking. He did not really say if he wanted formal instruction or if he wanted to have something more like on-the-job training or something on particular techniques? In any case, I can give him the information that I have, and see if it

Re: [Histonet] Advanced Histo training

Joelle, I agree that no program can be completed in 4 months, but they are not looking for a complete program. It would be nice to hear exactly what are they looking for with advance training, training with Immunos, special stains, etc.. Victor Victor Tobias Clinical Applications Analyst Un

[Histonet] RE: Dako and Leica immunostainers

No Way!! Just because there are new gadgets & gizmos on the newer instruments doesn't make them better. Versatility = Simplicity! Taking an instrument that works GREAT off the market just because there are newer ones that are all limited is dumb. We should follow Darwin and see the natural selec

RE: [Histonet] Advanced Histo training

I am not aware of any Histology program that can be completed in 4 months. I know that there are a few online programs which offer condensed training and instruction. I am involved with one located in Columbus, Ohio. But this program is 3 quarters in length plus prerequisites. We do offer some

[Histonet] immuno set-up

Hi, For small volumes like that, check with Thermo. They have various sizes of IHC stainers that have different slide capacities. Alternatively you could check out the Shandon Sequenza. I could find more info if you are interested. (BTW: I have no Thermo ties.) Amos Brooks Message: 19 Date: T

RE: [Histonet] Sakura tape problem

Sorry about the name. I meant Cheryl. TGIF __ From: "CHRISTIE GOWAN" <[EMAIL PROTECTED]> To: [EMAIL PROTECTED], [EMAIL PROTECTED] Subject: RE: [Histonet] Sakura tape problem Date: Fri, 21 Nov 2008 19:35:49 +

[Histonet] Advanced Histo training

Is there anyone out there that can answer this gentleman's questions. He is interested in getting advanced training in histotechnology over a 4 month period. We offer an AS degree and the HT classes are spread out over 2 years. Thank you, hi Sir, I am khalid al-housni from Oman working as h

RE: [Histonet] Re: bluing hematoxylin and alkaline water????

This is why I love the histonet!! -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Robert Richmond Sent: Friday, November 21, 2008 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: bluing hematoxylin and alkaline water Hard water (w

[Histonet] Re: bluing hematoxylin and alkaline water????

Hard water (water containing dissolved calcium carbonate and/or sulfate is alkaline enough to use as a bluing agent by itself. New York City, Hot Springs Arkansas, and San Antonio Texas - in my personal experience - have tap water sufficiently alkaline that you can blue hematoxylin in it in a reaso

RE: [Histonet] Sakura tape problem

Ah - sorry, didn't read that well. Nevermind. "Weems, Joyce" <[EMAIL PROTECTED]> 11/21/2008 01:36 PM To "Jackie M O'Connor" , "Cheryl Crowder" <[EMAIL PROTECTED]> cc <[EMAIL PROTECTED]>, "Histonet" <[EMAIL PROTECTED]> Subject RE: [Histonet] Sakura tape problem How do you do thi

RE: [Histonet] Sakura tape problem

How do you do this with the tissue attached to the tape? Just curious... j -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jackie M O'Connor Sent: Friday, November 21, 2008 2:32 PM To: Cheryl Crowder Cc: [EMAIL PROTECTED]; Histonet Subject: Re: [Histonet]

RE: [Histonet] Sakura tape problem

__ Cherly, I had this same problem a while back with an outside slide that had been sent to us for consultation. I even called the company to get any input from them. No luck. My final solution was to trim the tape do

Re: [Histonet] Sakura tape problem

I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' "Cheryl Crowder" <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 11/21/2008 01:20 PM To "Histonet

[Histonet] Sakura tape problem

Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or c

[Histonet] help

The chicken came first--but she did not have a belly button... Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN

[Histonet] Human tissue for research purposes

Human tissue for research purposes can be rather complicated. Keep in mind, your institution has requirements, the FDA has requirements and the patient has requirements. If you get the tissue from another institution...you guessed it, more requirements. I have worked with Institutional Revie

[Histonet] Floaters

Would anyone care to share their policy on floaters? Also, does anyone know if there is a CAP policy in floaters? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot [EMAIL PROTECTED] 989.463.1101 ext 3423 Please note that this email message and any attachments may

[Histonet] TFE3 testing

Does anyone do TFE3 gene testing on renal cell CA in children? If so, do you accept cases for consultation? Can formalin-fixed, paraffin-embedded tissue be used? Billing mechanics? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pa

[Histonet] RE: Leica Slide Writer

Is the flash on your printer working? The ink will smear or wash off during staining if the flash does "cure" the ink. Teresa Wylie -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Thursday, November 20, 2008 7:16 AM To: histone

RE: [Histonet] Osteoid identifiction

Hi Louise, Yes, you are right best way to quantify the osteoid is plastic embedding (MMA) but recently I saw a paper in "Stain Technology" the title is "Silver Staining of bone prior to decalcification for quantitative determination of osteoid in sections" by E.J. Tripp and E.H. MaoKay, dept of pat

RE: [Histonet] Does anyone have the link for the free LCM webinar that was on histonet this week?

Christina, Enclosed is the LCM webinar Shakun Announcing MDS Analytical Technologies next in the series of Laser Capture Microdissection and Microgenomics Webinars. Our next LCM webinar will be held on Monday, November 24, 2008 at 10AM PST or 1PM EST. The speaker will be Charmain Pietersen, PhD.

AW: [Histonet] bluing hematoxylin and alkaline water????

Is this a Haematoxylin-Eosin-stain? If so, I think you wash out the Eosin during the long water-wash. That makes the difference. Haemalaun binds to negative charged tissueparts best in acid solution (pH3-4). With this pH the colour is redish. Blueing means to elevate the pH about 7-8. Now the boun

Re: [Histonet] bluing hematoxylin and alkaline water????

Eva: Hematoxylin is a pH indicator, it turns reddish when you differentiate it in the acid alcohol, and returns to "violet-blue" when you either "blue" it with the ammonia water or place it in tap water after the alcohol-acid solution. The differentiation you know is to make the nuclear details m

[Histonet] Blueing Hematoxylin

My lab does not blue the hematoxylin. Some say that it makes the nuclei stand out more, but you don't have to. You can sometimes over blue as well. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/list

[Histonet] bluing hematoxylin and alkaline water????

Good morning out in histoland, Thank god it is Friday. Have just a small question that I am sure you all have an answer for. It is about bluing slides after hematoxylin. I read somewhere that alkaline water can blue hematoxylin and I am wondering if this is part of our problem. We use an automa

[Histonet] Does anyone have the link for the free LCM webinar that was on histonet this week?

Does anyone have the link for the free LCM webinar that was on histonet this week? I'd like to register but have deleted the post from my e-mail and I can not find it in the archives yet. Thanks, Christina Thurby Bristol Myers Squibb 812-429-8097 _

[Histonet] Re:Control slide storage

Just as a frame of reference, alkaline phosphatase is a very labile enzyme that can loose activity quickly in storage. For alk phos histochemistry controls, we store fixed peripheral blood smears individually wrapped in parafilm at -70 C for up to a year with minimal loss of activity. Mark Fre

[Histonet] RE: tissue/formalin disposal

That is exactly how we dispose of our tissue. We decant off the formalin, de-formalize and test it and if it passes rinse down the drain. By the way I had to get NH state approval to do this. The tissue is bagged, boxed and then hauled off for disposal. Tom Podawiltz, HT (ASCP) Histology Secti

R: [Histonet] Anti-fluorophore antibodies (for MOM work)

I used converter POD from roche and it works good -Messaggio originale- Da: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Per conto di Mikael Niku Inviato: venerdì 21 novembre 2008 13.42 A: histonet@lists.utsouthwestern.edu Oggetto: [Histonet] Anti-fluorophore antibodies (for MOM work) Dea

[Histonet] Anti-fluorophore antibodies (for MOM work)

Dear Histonetters, I'd like to ask for recommendations for anti-fluorophore antibodies. I'm planning to use a fluorophore-labelled primary antibody for mouse-on-mouse IHC. This is then detected using a labelled anti-rabbitIg (or whatever host it is) ab. Apparently Dako has discontinued the unlabe

RE: AW: [Histonet] Control slide storage

I feel that as far as surgical human samples that were originally taken for a quick diagnoses( which means minimal NBF fixation) with a just [EMAIL PROTECTED], the first 50-100u into the block is fine, after that who knows how well it is fixed/processed and this is where

Re: AW: [Histonet] Control slide storage

I'd love to know why and how, because I see it regularly. A definite fading of staining intensity on identical, but older, sections. I notice it with CD10, among others. Piero Nelva ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http: