Re: [Histonet] lids for stainer containers

These sound like the kind of thing that goes on a foil "take away" or freezer container. Try a home suply depot? We have specialist stores here that supply serious home bakers and chocolatiers and I know that they carry what you describe - bit far for you to come though... On 12/3/08, Per

Re: [Histonet] Alias identity

Here's one potential problem with listing real names: a few years ago a very diligent student of mine inadvertently stirred up a lot of controversy when she asked for advice from Histonet on a class assigment and someone was concerned that she was "cheating". I had in fact recommended Histone

[Histonet] PSA-NCAM IHC on frozen section with BrdU

Hi everyone, it seems that I lost the PSA-NCAM staining when sections are treated with citrate buffer (poring the membrane?) Possibly the membrane was dissociated from membrane then>? or? For co-staining with BrdU, I can only treat sections with HCl - the results sometimes are unstable~~ Anyone

Re: [Histonet] Blue haze

I thought that was Purple Haze, by Jimi Hendrix. Oh dear me, is it Friday yet? Forgive me, we are in the window for CAP and I'm just all in a haze. JTT - Original Message - From: "Histonet Alias" <[EMAIL PROTECTED]> To: "Marshall, Kimberly" <[EMAIL PROTECTED]> Cc: Sent: Wednesday, Dec

RE: [Histonet] GMA embedded section attachment to slides

Jim, Is it possible for you to provide more information as to the type of specimen you are working with and how thick you are cutting the sections? Jack> Date: Mon, 1 Dec 2008 16:26:31 -0600> From: [EMAIL PROTECTED]> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] GMA embedded sec

[Histonet] JCAHO

FYI Everyone: If you are still waiting for your inspection, don't bother putting up Christmas decorations. Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Blue Haze

I also use the Surgipath SelecTech H&E system and had to solve the blue haze problem in our lab. We've had great success simply using warm tap water to wash the slides after hematoxylin, rather than cold tap water. Hope that helps! -- Jennifer L. Springsteen, B.S. Lab Manager, Assistant Scienti

RE: [Histonet] IHC on paraformaldehyde-fixed

Gee I hate the term paraformaldehyde (as many of you probably know) This is an example of how confusion of terms can cause unnecessary work. Is "4% paraformaldehyde" different from 4 % formaldehyde? No Should any procedure done to tissues fixed in "4% paraformaldehyde" give results different to

RE: [Histonet] Blue haze

Excuse my ignorance but what is a clarifier step in association with the H&E stain? Or do you mean the differentiation step? Also having been a member of the marvelous Histonet server for many years I think I know who the Histonet Alias is!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys

RE: [Histonet] IHC on paraformaldehyde-fixed

I do it all the time. Yes your antigen-retrieval method will work. Ruth N.I.H. -Original Message- From: Jan Shivers [mailto:[EMAIL PROTECTED] Sent: Wednesday, December 03, 2008 4:34 PM To: histonet Subject: [Histonet] IHC on paraformaldehyde-fixed Has anyone ever done IHC on parafomald

[Histonet] IHC on paraformaldehyde-fixed

Has anyone ever done IHC on parafomaldehyde-fixed tissues, and if so, how well did it work? Will the same antigen-retrieval methods used with formalin-fixed tissue be applicable? I'm asking for an investigator, who already has his tissues fixed in paraformaldehyde. Jan Shivers Senior Scientis

[Histonet] Please remove from mailing list

Please remove me from the Histonet mailing list. Thanks for the valuable information I've received through this outreach. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] What is the name of this blue stain?

monastral fast blue "Madary, Joseph" <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 12/03/2008 11:15 AM To cc Subject [Histonet] What is the name of this blue stain? Alcian Blue goes into an alkaline alcohol for one hour as part of the old style movat. What is the final name of th

[Histonet] IHC billing with Mohs

If there is anyone on this list knowledgeable about billing of Mohs samples, I'd be grateful if you would contact me back channel to address one or two questions. Thank you, Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Sui

Re: [Histonet] What is the name of this blue stain?

Monastral fast blue, an insoluble pigment Rena Fail -- Original message from "Madary, Joseph" <[EMAIL PROTECTED]>: -- Alcian Blue goes into an alkaline alcohol for one hour as part of the old style movat. What is the final name of that blue color? Trying to train

[Histonet] What is the name of this blue stain?

Alcian Blue goes into an alkaline alcohol for one hour as part of the old style movat. What is the final name of that blue color? Trying to train someone and could not find it. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory Mgr One Medimmune Way, Lab 2438-Area 4 Gaithersburg, M

[Histonet] Flame substitute for embedding

This sounds like a similar problem we had in Microbiology decades ago when we had to stop using open flame for sterilizing our wire inoculating loops when streaking Petri plates. Most micro labs now use electric sterilizers which consists of a tightly wound heating coil that the wire loop is p

RE: [Histonet] Alias identity

This is one problem you have with an open list. I also belong to the Pathologists' Assistants list server but you must apply for membership that is restricted to AAPA members only. We can be more open with comments and questions without worry of unwanted calls from vendors or junk mail from the g

[Histonet] 2x3 slides boxes

Miss read submission but was able to find 2x3 slide boxes at VWR Micro Slide Boxes, Wood Frame Supplier: Jordan Paper Box Find Similar Items in Product CategoryStorage, Boxes, Plates, and Racks Supplier: Jordan Paper Box Micro Slide Boxes, Wood Frame This durable box can hold up to 100 stan

Re: [Histonet] Blue haze

On Wed, Dec 3, 2008 at 12:47 PM, Marshall, Kimberly < [EMAIL PROTECTED]> wrote: > Howdy all, > > I have just changed over to Surgi Path's H & E system. With the latest > fear of running out of hematoxylin and all my Pathologist wanted us to > change over. Its a great stain but we have a bad "h

[Histonet] RE: Blue haze

We tried the Surgipath H/E and had a similar experience. We went back to what we had been using. Since then I've been told that more water rinses or a different Clarifier type reagent may fix this problem, but I haven't had time or a good reason to give it another try. Ross M Stapf Histopatho

[Histonet] Thanks & new labeler question

Thanks to all for the answers on my expired reagents. Now, I have a new question. Is anyone using the Brady label printer or another similar printer for slide labels? I would be interested in hearing your experiences. Thanks, as always. Patti Loykasek This e-mail message, including any attachm

Re: [Histonet] Blue haze

Hi Kimberly, Are you using a clarifier step? On Wed, Dec 3, 2008 at 12:47 PM, Marshall, Kimberly < [EMAIL PROTECTED]> wrote: > Howdy all, > > I have just changed over to Surgi Path's H & E system. With the latest > fear of running out of hematoxylin and all my Pathologist wanted us to > change

[Histonet] formailin dispensing (large volumes)

Hi Histonetters, Can anyone share anything about their formalin preparation and dispensing? We are fortunate enough to be planning for a new morgue. In our existing morgue, we manually make 200L of formalin (from concentrate). It is made in a large tank and then pumped up into the upper tan

[Histonet] Re: PLP fixative lysine

For the PLP fixative, the full name for the lysine is L- lysine monohydrochloride. Sigma cat# L5626. Gayle M. Callis HTL(ASCP)HT,MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Blue haze

Howdy all, I have just changed over to Surgi Path's H & E system. With the latest fear of running out of hematoxylin and all my Pathologist wanted us to change over. Its a great stain but we have a bad "haze" in the background. It is not hurting the tissue or DX at all, but to ME it loo

RE: [Histonet] RE: Flame substitute for embedding

We have a Shandon Paratrimmer, which melts the wax not only off blocks but does a good job of keeping those forceps clean and hot. We have a bacti-incinerator, but know we can get a tall flame if we leave the paraffin coated forceps in there!! Bernice Bernice Frederick HTL (ASCP) Northwestern Uni

[Histonet] lids for stainer containers

We are looking for a source that sells a lid that looks like wax covered cardboard. We use them on top of the Shandon slide containers holding xylene or formula 83 while waiting to coverslip. They are about 5x7 and very thin. They have been here for years so I have no idea where they came fro

Re: [Histonet] 2x3 slides

I got the slides from Thermo Fisher with some problems a long while ago.  The issue was finding a 100 slide box for storage and shipping.  I did order them this morning from Brain Research and they had stock of the boxes.  Thanks to everyone who helped and now I know where to go for the boxes

[Histonet] 2x3 slides

Fisher has them MILLIPORE CORP MICROSCOPE SLIDES 2"X3" 72/PK Fisher offers many products that do not appear in our catalogs. This may be one of those products, so pictures and detailed descriptions are not available. However, you may be able to order it by adding it to your shopping cart. It

[Histonet] Shandon Processor

Any one in histoland using the Shandon Excelsior? How long have you had it & how many blocks can it process at a time? Cindy DuBois Integrated Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/

Re: [Histonet] IHC on fresh frozen

After hearing a presentation by Sharon Lear describing some low temp antigen retrieval that she did, we changed our method for frozen sections. We fix the frozen sections in 10% nuetral buffered formalin for 30'-60', rinse, then do a gentle pretreatment. The gentle pretreatment is usually 10mM citr

[Histonet] Re: Fungus staining with Warthin Starry

There is no oxidizing step in Warthin Starry to prepare fungal walls for silver deposition. This stain is for spirochetes. I suggest you use the Grocotts (Gomori's modification) methenamine silver where chromic acid is used to oxidize the fungal cell walls. You can find this on the web, or

[Histonet] Detailed Re: Attaching GMA sections to slides

Jim, You did not say what you were doing to the sections after mounting them on slides as this may be a factor for section lifting off other than It is important to have perfectly flat sections, so make sure the blades or glass knife is very sharp. GMA is designed for use for thin sections

[Histonet] RELIA Histology Management Job Alert 12/03/08

Hi Histonetters!! I hope everybody had a great Thanksgiving. I have several clients that are in need of managers and supervisors so I thought I would put the word out. At this point since it is the end of the year my clients are willing to let you call the shots on how you would like to proceed i

RE: [Histonet] IHC on fresh frozen

I was taught to get frozen sections into cold acetone immediately after sectioning, not letting the section air dry. We do C4d staining on frozen renal bxs and immediately fix the sections in cold acetone for 10 minutes. Then we air dry followed by 2 minutes in Ventana Morphosave (not a critical

[Histonet] Insulin/Glucagon double IHC

Dear Colleagues, I would like to ask your advices. I should establish double IHC staining on FFPE isolated mouse pancreatic islets for alpha and beta cells. I have very restricted amount of slides and I do not have possibility to test different antibodies and protocols. Could you please give me

[Histonet] Alias identity

I have to put my 2 cents in about all the statements on having an alias name instead of identifying who each person is after each email...WHAT'S THE BIG DEAL?? We are all supposed to be professionals asking each other for advice/suggestions on the Histonet - who cares who each person is? If I

[Histonet] Histology Supervisor position in Frederick, MD

Hello HistoNet - A facility I work with in Frederick, MD has asked for my assistance in finding a Histology Supervisor. Day shift, rotation of call on evenings and weekends. Salary range is $44,512 - $72,800 and relocation assistance available. If you or anyone you know would be interest

[Histonet] Histonet alias

It is not uncommon for many/most online forums to have users create screennames that they use for their online moniker. You never really know who they are so everybody, unless they want to divulge their identity, stays anonymous. How can we really know all of the registered names on the histonet

[Histonet] RE: Flame substitute for embedding

We also used to have an open flame while embedding! Now, we simply have a policy in place where after EACH cassette is embedded, the Techs MUST thoroughly wipe their forceps with disposable Kleenex (and then dispose of it!). Also, we use toothless forceps as much as possible to reduce the poss

RE: [Histonet] Flame substitute for embedding

We use forceps warmers, find them in any Histology catalog (like Fisher Scientific or Scientific Products/Allegiance). Janet L. Bonner, HTL (ASCP) Pathology Laboratory From: [EMAIL PROTECTED] on behalf of Celebre Julia Sent: Wed 12/3/2008 10:38 AM To: histon

[Histonet] Flame substitute for embedding

Hello Histoland!! We have just been told we are no longer allowed to use alcohol burners while embedding and we are to find a safer substitute, something to do with no liking an open flame in the lab. Other than incinerators or 6 pairs of forceps at each centre, we've run out of ideas so we a

Re: [Histonet] Histo humor :-)

Or like an occasional chair? Geoff Joe Nocito wrote: is a casual histo tech like a casual end table? What is it the rest of the time? Oh, yeah, we not only have a great cents of humor, we're real cut ups to. JTT - Original Message - From: "Dianne Holmes" <[EMAIL PROTECTED]> To: "H

[Histonet] IHC on fresh frozen

Hi, all Recently I did some IHC(chromagen methods) on mouse fresh frozen tissues, mainly using insulin antibody on pancreas. The image is much fuzzier compare to paraffin embedding tissue. And the staining also smeared to acinar cells which surround the islet. I airdried slide(>30min) and

[Histonet] Biomeda Corp.

All, I vaguely remember a discussion on this, but could someone please refresh me on who bought out Biomeda and perhaps provide contact information for whomever is now dispensing their product? I am looking for their FITC Streptavidin (F72) so if there are any vendors that provide a similar

Re: [Histonet] Ventana Benchmark XTs

Let's agree to disagree here. I will continue to post when I feel necessary and the people who don't agree with my reasoning can just ignore me. Just for the record I do not work for any vendor and I am a Histology Manager for a private laboratory in the midwest. Will my name make any difference in

[Histonet] Lysine

Good Morning Histonetters, Can anyone tell me of a supplier and specific grade of L-lysine HCl for the make up of 2% periodate lysine paraformaldehye fixative? Thank you. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517

RE: [Histonet] RE: Casual Histotech

Who knew? Thanks! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Laurie Popp Sent: Wednesday, December 03, 2008 7:40 AM To: histonet@lists.utsouthwestern.edu Subj

[Histonet] RE: Casual Histotech

Casual Histotech is usually considered to either be very part time or a pool position to cover only when needed. Laurie Popp, BA HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/hi

[Histonet] RE: Large Slide Boxes

Hello Pam, We carry several types of slide boxes for 2"x3" slides as well as boxes to hold slides up to 5"x7". Please feel free to call me toll-free @ 1(888)275-5544. Nora Bender Brain Research Laboratories Tel: 617-965-5544 www.brainresearchlab.com Goo