RE: RE: RE: [Histonet] IHC on paraformaldehyde-fixed

Isn't petrol toxic? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don’t be afraid to take a big step when one is indicated. You can’t cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and

[Histonet] thermometer calibration

Hi All, Just wondering how everyone is doing their thermometer calibrations. Inspection time is right around the corner so I want to be ready! Thanks. Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are

[Histonet] Marketing Position

We are currently recruiting for a marketing position in our organization. We are an Anatomic Pathology reference lab located in Lakeland, Florida and are looking for someone with both lab and marketing experience. If you are interested or know someone who is, please contact me toll-free at

[Histonet] Paul Monfils re: PMMA blocks

Paul, Rather than add to Jack and Gail's experience and expertise, feel free to call me if you need additional hints. I have no technical problems with large PMMA blocks. One hint is to use Pyrex beakers or crystallizing dishes, although expensive, the results are consistent. Best regards,

RE: [Histonet] PFA preparation

We routinely add paraformaldehyde to alkaline water at room temperature while stirring and wait only about 30-60 mintues for it to dissolve. Then we add a concentrated amount of PBS up to the total required volume (so that the buffer is 1x in the final volume). Then we add acid to bring the

RE: [Histonet] PFA preparation: Does dissolution also mean that the PFA has depolymerized?

Hi Merced, Thanks for your reply. So then you manipulate pH rather than temperature to ensure the dissolution of PFA. Does dissolution also mean that the PFA has depolymerized? Are you saying heating to 60 degrees C is not required, or that it is not recommended? I've been told that heating

[Histonet] processing times

Hello-I have just started at Roswell Park Cancer Institute and we are hoping to decrease our TAT by decreasing our processing times-having come from a facility where we hadn't changed processor times in 20 years I would appreciate it if someone could supply their schedules so I could do a

Re: [Histonet] microwave processor

The Pathos can only run one run at a time. It does everything a conventional processor does. It is fully automated. On Mon, Dec 8, 2008 at 7:50 AM, Evans, Andria B. [EMAIL PROTECTED]wrote: We are currently looking at getting another microwave processor. We currently have the Histos 5 and are

Re: [Histonet] Silanized slides

The solution for making silanized slides (APES in acetone; also needs a trace of water, which does not need to be added specially) has to be used right away: it's stable for perhaps a few hours. The resulting slides, washed and dried, should be stable indefinitely if kept clean. See Microscopy

Re: [Histonet] Alias identity

Amber McKenzie [EMAIL PROTECTED] wrote: . . .We are all supposed to be professionals asking each other for advice/suggestions on the Histonet - who cares who each person is? If I post a question, I don't care if it's Jane Doe answering or John Smith ... That's true, and it certainly makes

RE: [Histonet] Alias identity

While I prefer to go by my own name, I recognize that some people rightly fear to do so. An anonymous source may still have something worthwhile to say. Experience does not always teach well. The wise and experienced Linus Pauling and Erwin Chargaff were wrong about the structure of DNA; the

Re: RE: [Histonet] PFA preparation

We routinely add PFA to 0.1M PBS and heat, stir the solution for 2-3 hours. Longer heating will result in acidic pH - depolymerizing..? Then we adjust the pH to 7.4 2008-12-09 tf 发件人： Merced Leiker 发送时间： 2008-12-09 00:05:52 收件人： Tony Henwood; [EMAIL PROTECTED];