I was wondering if anyone has tried Isopropyl Alcohol (2-propanol) as an
alternative to xylene substitutes in their tissue processors. We are
attempting this method with the Thermo Excelsior to process mouse embryo,
placenta, liver, and mouse and rat organs. Currently our animal tissue has be
I used to be in technical support for a chemical company EMD Chemicals
which manufactures pH test strips. If I remember correctly, they were
not expiration dated. There are conditions that can cause errors. I
attached a brochure that states the errors on page 6. You should keep it
for you record
Another option:
When you fix overnight, is that with the colon opened and in fixative, or
with the tissue in cassettes in the fixative overnight? That should be fine.
Or is that placing the unfixed colon intact into the fixative overnight? If
it is this way, then the fixative has not penetrated t
First I would determine if it is really DNA streaming out of the nuclei. Stain
the sections with the Feulgen-Rossenbeck method, then I would also review the
speed of freezing the tissue before FS, usually freezing time produce many
artifacts in the final sections.
René J.
--- On Sun, 1/4/09, P
Hello:
I have recently begun to use frozen sections to localize an antigen found in
mouse liver tissue. My previous experience has been almost exclusively with
staining of formalin-fixed, paraffin-embedded tissues. I have done two runs of
this new staining protocol and noticed an odd artifa
There is definitely a difference in selecting the matching isotype of
secondary for IHC. Many people learned that lesion back in the day trying
to stain for Hodgkins with CD15 which was IgM, we would get negative results
because the secondary reagents were just recognizing IgG. It is my
understan
As Ray states it is all empirical, some will work from WB to IHC and some
will not in my experience. In general if the spec sheet recommends a
dilution for WB at say 1:2000, I will start using the ab for IHC on tissue
at 1:200 (it will definitely need to be tittered and used at probably around
10
Demo them all then decide which one works best for you.
On Sat, Jan 3, 2009 at 7:42 AM, kristen arvidson
wrote:
> Hello all,
>
> I am looking for info on IP stainers. Which stainers are people using?
> What does everyone like, dislike, etc. Any info is helpful. Thanks!!
>
> Kristen
>
>
>
> ___
Most definitely that could be the problem. A colon in the refrigerator,
although it will not be rotten, the cellular detail will be affected.
You have to coordinate with the OR for the colon to be brought to histology and
start fixing immediately, anything else will affect the quality of the
mic
