[Histonet] texas red

2009-01-16 Thread ejschmid
I need my GMA plastic sections to fluoresce, that is the plastic itself. For the embedded tissue itself, I am using DAPI and Alexa Fluor 488. So the stain I choose for the plastic, which I need to see using fluorescence, cannot interfere with the DAPI or Alexa Fluor 488. This is basically a

[Histonet] PhosStop and Complete and cryosections

2009-01-16 Thread Kim Merriam
Hi Everyone,   Happy Friday!   I am looking to use PhosStop and Complete (both from Roche) in my fixative for cryosections (and cytospins).  I have used in in the past in formalin for FFPE xenografts, and now I want to use it as a cryo-section fixative.  What I want to know is: has anyone

[Histonet] NC Society for Histopathology Technologists 2009 Meeting

2009-01-16 Thread James L Burchette
The North Carolina Society for Histopathology Technologists invites you to our 2009 meeting to be held May first and second at the DoubleTree Biltmore Hotel in beautiful Asheville North Carolina. Call the DoubleTree Biltmore Hotel at 1-800-222-8733 for special room rates and reservations or

[Histonet] Methylene Blue

2009-01-16 Thread anitaibsc
Hello Netters, We are looking for a recipe and instructions for Methylene Blue counterstain and histology reagent, can anyone help. Thank you. Anita Email: anitai...@aol.com www.ImmunoBioScience.com ___ Histonet mailing list

Re: [Histonet] Methylene Blue

2009-01-16 Thread Rene J Buesa
Anita: There are scores of formulas using methylene blue, but the majority use what is called a saturated solution which is about 4.5%  aq. sol. Regarding the action time it is better for you to test some times and select the one is more suitable for your purposes although it is usually used

RE: [Histonet] New Dako IHC Instrument and T.P.I.D.

2009-01-16 Thread Sally Price
Beth: I evaluated the AS-Link/TPID and wasn't too impressed. Its the same basic machine with a new outside/skin and the only difference is that in can read barcode labels on the reagents. To me, the postive-ID part should be handles by the LIS, not the staining system. One part of the deal that