Re: [Histonet] double staining on the ventana XT

2009-01-23 Thread Piero Nelva
We also run a p63/HMCK/p504s dual stain cocktail on prostate cores. Easy to set up and results look great. THe Kappa/Lambda dual stain works, too. I find the red chromagen (lambda) in this setting is a little less precise, tho'. REgards Piero Nelva Anatomical PAthology Monash Medical

RE: [Histonet] RE: Prion Contamination

2009-01-23 Thread Bartlett, Jeanine (CDC/CCID/NCZVED)
All, The formic acid is a post-fixation step. http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s7d.htm#Table%205.%20Tissue %20preparation Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114

RE: [Histonet] Re: Xylene substitute

2009-01-23 Thread Blazek, Linda
Dear Bob Richmond, our honorable Samurai Pathologist. The consensus of opinion around here is that you have been spending too much time smelling formalin! We can't figure out how you get the smell of ether from Formula 83! Most of us here are old enough to remember the smell of ether but

[Histonet] Cytology Autostainers

2009-01-23 Thread Greg Dobbin
Hi Folks, What's out there? What's hot and what's not? Anyone using their cytology autostainer for histology special stains? Welcome all input (including vendors). Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O.

[Histonet] RELIA Special Job Alert Brand New Management and Supervisory Opportunities 1-23-09

2009-01-23 Thread Pam Barker
Hi Histonetters, I have several brand new exciting opportunities for experienced Managers,and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and

Re: [Histonet] Re: Xylene substitute

2009-01-23 Thread Victor Tobias
In defense of the good Samurai, he was using a comment posted by someone else that the product smelled like ether. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195

[Histonet] Legal Question

2009-01-23 Thread Jessica Piche
Hi, We have an antibody that we ordered in error. We would like to see if another lab would like to purchase it from us. Is that legal to ask this on the Histonet? Thanks!   Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list

Re: [Histonet] Legal Question

2009-01-23 Thread Jackie M O'Connor
Or you can just ask the supplier to take it back. Jessica Piche jessgro...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 01/23/2009 11:38 AM To histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] Legal Question Hi, We have an antibody that we ordered in

[Histonet] (no subject)

2009-01-23 Thread MARY T HODGES
Good day all, Thank you so much for your input on decal billing. I have another questain? This one is a little strange. When you put in charges as a prelimary charge ... do you over bill or under bill? I have always stayed under while putting in charges and then audit the doctors charges

Re: [Histonet] Starfrost slides

2009-01-23 Thread Merced Leiker
I have used them almost daily for a year or more now and have had no problems with the slides giving off any autofluorescence in immunofluorescence applications. --On Thursday, January 22, 2009 2:56 PM -0500 Nancy Lemke ns...@neuro.hfh.edu wrote: Has anyone using Starfrost slides (7255)

[Histonet] LabVision/Dako Autostainer

2009-01-23 Thread Della Speranza, Vinnie
We have earlier generation Dako Autostainers and are evaluating a current generation LabVision stainer. New or old we have experienced occasions when this instrument design has failed to dispense reagent. This seems to occur randomly or if there is a pattern we haven't figured it out. I've

Re: [Histonet] Starfrost slides

2009-01-23 Thread Jennifer Harvey
From what I understand they are made with green glass. Green glass is a less superior glass than white and you can have fluorescence issues. You should only use white glass for fluorescence applications. You can tell by looking at the slides in the box on their sides. The Mercedes slides (7255)

[Histonet] Ventana Basic V-Red Kit

2009-01-23 Thread Cindy . J . Chard-Bergstrom
How many labs out there are still using the basic V-Red Kit from Ventana? Have you had any problems using the enhanced or the ultraview red on animal tissues? Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] LabVision/Dako Autostainer

2009-01-23 Thread CHRISTIE GOWAN
Hey Vinnie, We have 2 lab visions and we also experienced this problem. They came in and changed the level sensors and we have not had the problem since. Christie UAB Hospital Birmingham, AL From: del...@musc.edu To: histonet@lists.utsouthwestern.edu Date: Fri, 23 Jan 2009 15:13:36 -0500

[Histonet] RE: Prion Contamination-Post Fixation

2009-01-23 Thread Terri Braud
Ooops, my bad!..you are both right! Another reason that prion contaminated tissues should be left to the experts. And for the rest of us, just read the links for yourself, and try to be safe! Thanks, Terri Terri L. Braud, HT(ASCP) My recollection I believe that it stated that the formic

[Histonet] methyl green - multiple labelling

2009-01-23 Thread anjan kumar
hi everybody, has anyone tried methyl green as counter stain in multiple label immunohistochemistry. i tried one but i got a dull back ground when i took a snap through Image pro plus 5.1. regards, anjan. _ For

Re: [Histonet] methyl green - multiple labelling

2009-01-23 Thread TF
yes methyl blue methyle green are too dim.. try neutral red...? it seems fast green/light green might lead to intense staining? I am not sure, you may test it. 2009-01-24 TF 发件人: anjan kumar 发送时间: 2009-01-24 13:11:19 收件人: triple immunohistochem 抄送: 主题: [Histonet] methyl green -